EpCAM Antibody [KS1/4], Rabbit IgG

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ProSci
Product Code: PSI-24-819
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-24-819-0.2mg0.2mg£681.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG kappa
Antibody Clonality: Monoclonal
Antibody Clone: KS1/4
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Flow Cytometry
Shipping:
Blue Ice
Storage:
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Images

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<b> Immunofluorescence staining of Caco-2 cells with anti_EpCAM (KS1/4 </b> Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells stained with the chimeric r version of KS1/4 (24-819) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-819- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.
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<b> Flow cytometry using the Anti-EpCAM antibody KS1/4 ( </b> Paraformaldehyde fixed Caco-2 cells were stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r version of KS1/4 (24-819- blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
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<b> Immunofluorescence staining of Caco-2 cells with anti_EpCAM (KS1/4 </b> Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells stained with the chimeric r version of KS1/4 (24-819) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-819- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.
<b> Flow cytometry using the Anti-EpCAM antibody KS1/4 ( </b> Paraformaldehyde fixed Caco-2 cells were stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r version of KS1/4 (24-819- blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Documents

Further Information

Additional Names:
CD326, Ep-CAM, KS1/4 antigen, KSA, Epithelial cell adhesion molecule, Adenocarcinoma-associated antigen, Cell surface glycoprotein Trop-1, Epithelial cell surface antigen, Epithelial glycoprotein, EGP, Epithelial glycoprotein 314, 17-1A-antigen, EGP314, hEGP314, Major gastrointestinal tumor-associated protein GA733-2, Tumor-associated calcium signal transducer 1.
Buffer:
PBS with 0.02% Proclin 300.
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
The original antibody was generated by immunizing BALB/c mice with human adenocarcinoma of the lung cell line UCLA P3.
NCBI Gene ID #:
4072
NCBI Official Name:
epithelial cell adhesion molecule
NCBI Official Symbol:
EPCAM
NCBI Organism:
Homo sapiens
Research Area:
Cancer
Swissprot #:
P16422
User NOte:
For research use only.