CD171 Antibody [L1-14.10], Mouse IgG1

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ProSci
Product Code: PSI-24-721
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-24-721-0.2mg0.2mg£681.00
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Overview

Host Type: Mouse
Antibody Isotype: IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: L1-14.10
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Shipping:
Blue Ice
Storage:
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Images

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<b> Western Blot using anti-CD171 antibody L1-14.10 ( </b> HeLa(A) (0.03 ug/ml)- Kelly(B) cells (0.01 ug/ml)- human brain cerebellum(C) (0.003 ug/ml) and cerebral cortex(D) (0.01 ug/ml) tissue lysate (35ug protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric mouse IgG version of L1-14.10 (24-721) before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
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<b> Flow cytometry using the Anti-CD171 antibody L1-14.10 ( </b> Paraformaldehyde fixed HeLa cells were stained with anti-unknown specificity antibody (.1; isotype control- black line) or the mouse IgG1 version of L1-14.10 (24-721- blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
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<b> Western Blot using anti-CD171 antibody L1-14.10 ( </b> HeLa(A) (0.03 ug/ml)- Kelly(B) cells (0.01 ug/ml)- human brain cerebellum(C) (0.003 ug/ml) and cerebral cortex(D) (0.01 ug/ml) tissue lysate (35ug protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric mouse IgG version of L1-14.10 (24-721) before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
<b> Flow cytometry using the Anti-CD171 antibody L1-14.10 ( </b> Paraformaldehyde fixed HeLa cells were stained with anti-unknown specificity antibody (.1; isotype control- black line) or the mouse IgG1 version of L1-14.10 (24-721- blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Documents

Further Information

Additional Names:
L1CAM, 14.10, L1, Neural cell adhesion molecule L1, N-CAM-L1, NCAM-L1
Buffer:
PBS with 0.02% Proclin 300.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
This antibody was raised by immunising mice with recombinant L1-Fc fusion protein, consisting of the ectodomain of human L1.
NCBI Gene ID #:
3897
NCBI Official Name:
L1 cell adhesion molecule
NCBI Official Symbol:
L1CAM
NCBI Organism:
Homo sapiens
Research Area:
Cancer
Swissprot #:
P32004
User NOte:
For research use only.