CD27 Antibody [LG.3A10], Rabbit IgG

ProSci
Product Code: PSI-24-473
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-24-473-0.2mg0.2mg£681.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG kappa
Antibody Clonality: Monoclonal
Antibody Clone: LG.3A10
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Activity Assay
  • Flow Cytometry
  • Immunofluorescence (IF)
  • Immunoprecipitation (IP)
Shipping:
Blue Ice
Storage:
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Images

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<b> Immunofluorescence staining of fixed mouse splenocytes with anti-CD27 antibody LG.3A10 (</b> Immunofluorescence analysis of paraformaldehyde fixed mouse (<i>Mus musculus</i>) splenocytes on Shi-fix™ coverslips- permewith 0.15% Triton stained with the chimeric rsion of LG.3A10 (24-473) at 10 ug/ml for 1h followed by Alexa Fluor®?488 secondary antibody (1 ug/ml)- showing membrane and some cytoplasmic staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-473- DAPI- merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody (0) followed by Alexa Fluor®?488 secondary antibody.
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<b> Immunofluorescence staining of fixed mouse splenocytes with anti-CD27 antibody LG.3A10 (</b> Immunofluorescence analysis of paraformaldehyde fixed mouse (<i>Mus musculus</i>) splenocytes on Shi-fix™ coverslips- permewith 0.15% Triton stained with the chimeric rsion of LG.3A10 (24-473) at 10 ug/ml for 1h followed by Alexa Fluor®?488 secondary antibody (1 ug/ml)- showing membrane and some cytoplasmic staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-473- DAPI- merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody (0) followed by Alexa Fluor®?488 secondary antibody.

Further Information

Additional Names:
TNFRS7, CD27L receptor, T-cell activation antigen CD27, Tumor necrosis factor receptor superfamily member 7
Buffer:
PBS with 0.02% Proclin 300.
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Hamster IgG format, for improved compatibility with existing reagents, assays and techniques.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
This antibody was produced by injecting Armenian hamsters intraperitoneally with a mixture of two murine CD27 tag-expressing Armenian hamster fibroblast clones (AR0524 and AR0530) in Freund's adjuvant
NCBI Gene ID #:
21940
NCBI Official Name:
CD27 antigen
NCBI Official Symbol:
Cd27
NCBI Organism:
Mus musculus
Research Area:
Immunology, Chemokines & Cytokines
Swissprot #:
P41272
User NOte:
For research use only.