IgM Antibody [M15/8], Rabbit IgG

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ProSci
Product Code: PSI-24-297
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-24-297-0.2mg0.2mg£681.00
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Overview

Host Type: Rabbit
Antibody Isotype: IgG kappa
Antibody Clonality: Monoclonal
Antibody Clone: M15/8
Regulatory Status: RUO
Target Species: Human
Applications:
  • Blocking
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Flow Cytometry
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunoprecipitation (IP)
Shipping:
Blue Ice
Storage:
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Images

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<b>ELISA of anti-IgM antibody on IgM Fc region peptide and a murine IgM antibody.</b> Binding curves of the rmeric version of the anti-IgM antibody M15/8 (24-297; red line) and isotype control (anti-Fluorescein atnibody; 3.0; blue line) to an ELISA plate coated with either IgM Fc region peptide (Pr00108-15.5) at a concentration of 5 ug/ml (A) or murine IgM antibody (B; Anti-CD41 antibody; 1.0) again at 5 ug/ml. A 3-fold serial dilution from 10-000 to 0.17 ng/ml was performed using 24-297. For signal detection- a 1:4000 dilution of HRP-lnti-r1 antibody was used. Both IgM Fc region peptide (monomeric ligand) and murine IgM antibody (multimeric ligand) were recognised by 24-297.
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<b> Western Blot using anti-IgM antibody M15/8 (</b> Human spleen lysate (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rsion of M15/8 (24-297) at 0.3 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for IgM is 180/900kDa for monomeric/pentameric IgM respectively. Here it is likely that the observed fragment is single glycosylated IgM heavy chain as s targetted to the Fc region of IgM (Cragg et al.- PMID: 10070880). 24-297 successfully detected IgM in human spleen lysate.
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<b>ELISA of anti-IgM antibody on IgM Fc region peptide and a murine IgM antibody.</b> Binding curves of the rmeric version of the anti-IgM antibody M15/8 (24-297; red line) and isotype control (anti-Fluorescein atnibody; 3.0; blue line) to an ELISA plate coated with either IgM Fc region peptide (Pr00108-15.5) at a concentration of 5 ug/ml (A) or murine IgM antibody (B; Anti-CD41 antibody; 1.0) again at 5 ug/ml. A 3-fold serial dilution from 10-000 to 0.17 ng/ml was performed using 24-297. For signal detection- a 1:4000 dilution of HRP-lnti-r1 antibody was used. Both IgM Fc region peptide (monomeric ligand) and murine IgM antibody (multimeric ligand) were recognised by 24-297.
<b> Western Blot using anti-IgM antibody M15/8 (</b> Human spleen lysate (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rsion of M15/8 (24-297) at 0.3 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for IgM is 180/900kDa for monomeric/pentameric IgM respectively. Here it is likely that the observed fragment is single glycosylated IgM heavy chain as s targetted to the Fc region of IgM (Cragg et al.- PMID: 10070880). 24-297 successfully detected IgM in human spleen lysate.

Documents

Further Information

Additional Names:
mu-chain
Buffer:
PBS with 0.02% Proclin 300.
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
BALB/c mice immunized with human IgM.
Research Area:
Immunology, Other
Swissprot #:
P01871
User NOte:
For research use only.