Cytokeratin 7 Antibody [OV-TL 12/30], Rabbit IgG

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ProSci
Product Code: PSI-24-802
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-24-802-0.2mg0.2mg£681.00
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Overview

Host Type: Rabbit
Antibody Isotype: IgG kappa
Antibody Clonality: Monoclonal
Antibody Clone: OV-TL 12/30
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
Blue Ice
Storage:
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Images

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<b> Immunofluorescence staining of fixed HeLa cells with anti-Cytokeratin 7 antibody OV-TL 12/30 (</b> Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix™ coverslips stained with the chimeric r version of OV-TL 12/30 (24-802) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-802- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.
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<b> Flow cytometry using the anti-Cytokeratin 7 antibody OV-TL 12/30 ( </b> HeLa cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r1 version of OV-TL 12/30 (24-802- blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
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<b> Immunofluorescence staining of fixed HeLa cells with anti-Cytokeratin 7 antibody OV-TL 12/30 (</b> Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix™ coverslips stained with the chimeric r version of OV-TL 12/30 (24-802) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-802- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.
<b> Flow cytometry using the anti-Cytokeratin 7 antibody OV-TL 12/30 ( </b> HeLa cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r1 version of OV-TL 12/30 (24-802- blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Documents

Further Information

Additional Names:
CK-7, Keratin, type II cytoskeletal 7, Cytokeratin-7, Keratin-7, K7, Sarcolectin, Type-II keratin Kb7, CK7, CK 7
Buffer:
PBS with 0.02% Proclin 300.
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
This antibody was originally raised by immunizing BALB/c mice with ovarian carcinoma cell line OTN11.
NCBI Gene ID #:
3855
NCBI Official Name:
keratin 7
NCBI Official Symbol:
KRT7
NCBI Organism:
Homo sapiens
Research Area:
Cancer, Other
Swissprot #:
P08729
User NOte:
For research use only.