MHC II Antibody [P7/7], Rabbit IgG

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ProSci
Product Code: PSI-24-540
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-24-540-0.2mg0.2mg£681.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG kappa
Antibody Clonality: Monoclonal
Antibody Clone: P7/7
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Flow Cytometry
  • Immunohistochemistry (IHC)
  • Immunoprecipitation (IP)
Shipping:
Blue Ice
Storage:
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Images

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<b> Flow-cytometry using the anti-MHC II antibody P7/7 ( </b> Mouse splenocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control- black line) or the r1 version of P7/7 (24-540- blue line) at a dilution of 1:100 for 1h at RT. After washing- bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
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<b> Flow-cytometry using the anti-MHC II antibody P7/7 ( </b> Mouse splenocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control- black line) or the r1 version of P7/7 (24-540- blue line) at a dilution of 1:100 for 1h at RT. After washing- bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Documents

Further Information

Additional Names:
P7.7, I-A, Ia, I-E, MHC-II, MHC Class II
Buffer:
PBS with 0.02% Proclin 300.
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
This antibody was raised by immunising DA rats with PVG.R8 rat spleen cells. Splenocytes from immunised rats were then isolated, and fused to the myeloma line NSO/u to generate stable hybridomas.
Research Area:
Other
User NOte:
For research use only.