p53 Antibody [PAb421], Rabbit IgG

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ProSci
Product Code: PSI-24-180
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-24-180-0.2mg0.2mg£681.00
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Overview

Host Type: Rabbit
Antibody Isotype: IgG kappa
Antibody Clonality: Monoclonal
Antibody Clone: PAb421
Regulatory Status: RUO
Target Species:
  • Human
  • Monkey
  • Mouse
  • Rabbit
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Immunoprecipitation (IP)
  • In Vivo Assay
  • Western Blot (WB)
Shipping:
Blue Ice
Storage:
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Images

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<b> Flow-cytometry using the anti-p53 antibody P00142) </b> Jurkat cells were stained with unimmunized r antibody (black line) or the rmeric version of P-180- blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing- bound antibody was detected using anti-r JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.
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<b> Western Blot using anti-p53 antibody P00142) </b> A431 cell nuclear (A) and cytoplasmic (B) extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rsion of P-180) at 0.1 ug/ml before detection by an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for p53 is 43.7 kDa- though due to the high number of proline residues in this protein runs at a size of ~53kDa (c.f. Ziemer et al.- PMID: 7107651). 24-180 successfully detected both human nuclear and cytoplasmic p53.
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<b> Flow-cytometry using the anti-p53 antibody P00142) </b> Jurkat cells were stained with unimmunized r antibody (black line) or the rmeric version of P-180- blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing- bound antibody was detected using anti-r JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.
<b> Western Blot using anti-p53 antibody P00142) </b> A431 cell nuclear (A) and cytoplasmic (B) extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rsion of P-180) at 0.1 ug/ml before detection by an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for p53 is 43.7 kDa- though due to the high number of proline residues in this protein runs at a size of ~53kDa (c.f. Ziemer et al.- PMID: 7107651). 24-180 successfully detected both human nuclear and cytoplasmic p53.

Documents

Further Information

Additional Names:
Tumor suppressor p53, Cellular tumor antigen p53
Buffer:
PBS with 0.02% Proclin 300.
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original murine IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide corresponding to aa 371-380 of human p53.
NCBI Gene ID #:
3525
NCBI Official Name:
immunoglobulin kappa variable 1/OR1-1 (pseudogene)
NCBI Official Symbol:
IGKV1OR1-1
NCBI Organism:
Homo sapiens
Research Area:
Cancer
Swissprot #:
P04637
User NOte:
For research use only.