CD25 Antibody [PC-61.5.3], Rabbit IgG

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ProSci
Product Code: PSI-24-631
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-24-631-0.2mg0.2mg£681.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG lambda
Antibody Clonality: Monoclonal
Antibody Clone: PC-61.5.3
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Depletion
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Flow Cytometry
  • Functional Study
  • Immunofluorescence (IF)
  • Western Blot (WB)
Shipping:
Blue Ice
Storage:
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Images

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<b> Immunofluorescence staining of fixed mouse splenocytes with anti-CD25 antibody PC-61.5.3 (</b> Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix™ coverslips stained with the chimeric r version of PC-61.5.3 (24-631) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-631- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (.1) followed by staining with Alexa Fluor® 488 secondary antibody.
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<b> Immunofluorescence staining of fixed mouse splenocytes with anti-CD25 antibody PC-61.5.3 (</b> Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix™ coverslips stained with the chimeric r version of PC-61.5.3 (24-631) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-631- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (.1) followed by staining with Alexa Fluor® 488 secondary antibody.

Documents

Further Information

Additional Names:
IL2-RA, IL-2 receptor subunit alpha,L-2R alpha chain, IL-2R subunit alpha, Interleukin-2 receptor subunit alpha, p55, p55 chain, PC61, PC6153
Buffer:
PBS with 0.02% Proclin 300.
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
This antibody was raised by immunising rats with the B6.1 mouse cytotoxic T cell line, followed by fusing spleen cells with P3X63Ag8.653 myeloma cells.
NCBI Gene ID #:
16184
NCBI Official Name:
interleukin 2 receptor, alpha chain
NCBI Official Symbol:
Il2ra
NCBI Organism:
Mus musculus
Research Area:
Immunology, Cell Cycle, Chemokines & Cytokines
Swissprot #:
P01590
User NOte:
For research use only.