CD86 Antibody [PO.3], Rabbit IgG

ProSci
Product Code: PSI-24-593
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-24-593-0.2mg0.2mg£681.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG kappa
Antibody Clonality: Monoclonal
Antibody Clone: PO.3
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Blocking
  • Flow Cytometry
  • Functional Study
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
Blue Ice
Storage:
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Images

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<b> Immunofluorescence staining of fixed RAW264.7 cells with anti-CD86 antibody PO.3 (B467) (</b> Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix™ coverslips stained with the chimeric r version of PO.3 (24-593) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-593- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.

<b> Immunofluorescence staining of fixed RAW264.7 cells with anti-CD86 antibody PO.3 (B467) (</b> Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix™ coverslips stained with the chimeric r version of PO.3 (24-593) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-593- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.

Further Information

Additional Names:
B7-2, PO3, Activation B7-2 antigen, B-lymphocyte activation antigen B7-2, B-lymphocyte antigen B7-2, B7.2, CD28 antigen ligand 2, CD28LG2, CD86, early T cell costimulatory molecule-1, early T-cell costimulatory molecule 1, ETC-1, LAB72, MGC34413, T-lymphocyte activation antigen CD86
Buffer:
PBS with 0.02% Proclin 300.
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
This antibody was raised by immunising Sprague Dawley rats with a mouse B cell line, BCL1 and fusing immune splenocytes with P3U1 myeloma cells.
NCBI Gene ID #:
12524
NCBI Official Name:
CD86 antigen
NCBI Official Symbol:
Cd86
NCBI Organism:
Mus musculus
Research Area:
Immunology, Neuroscience
Swissprot #:
P42082
User NOte:
For research use only.