Fas Antibody [R-125224], Rabbit IgG

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ProSci
Product Code: PSI-24-527
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-24-527-0.2mg0.2mg£681.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG kappa
Antibody Clonality: Monoclonal
Antibody Clone: R-125224
Regulatory Status: RUO
Target Species: Human
Applications:
  • Activity Assay
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Flow Cytometry
  • Imaging
Shipping:
Blue Ice
Storage:
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Images

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<b> Flow-cytometry using the anti-Fas antibody R-125224 (</b> Jurkat cells were fixed using 2% PFA- permeusing 0.5% Triton and stained with unimmunized r antibody (MOPC-21; isotype control- black line) or the r-chimeric version of R-125224 (24-527- blue line) at a dilution of 1:100 for 1h at RT. After washing- bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
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<b> Immunofluorescence staining of fixed MCF7 cells with anti-Fas antibody R-125224 (</b> Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells permewith 0.15% Triton and stained with the chimeric mouse IgG1 version of R-125224 (24-527) at 10 ug/ml for 1h followed by Alexa Fluor®?488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-527- DAPI- merged channels and an isotype control. The isotype control was stained with an anti-unknown specificity antibody (0) followed by Alexa Fluor®?488 secondary antibody.
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<b> Flow-cytometry using the anti-Fas antibody R-125224 (</b> Jurkat cells were fixed using 2% PFA- permeusing 0.5% Triton and stained with unimmunized r antibody (MOPC-21; isotype control- black line) or the r-chimeric version of R-125224 (24-527- blue line) at a dilution of 1:100 for 1h at RT. After washing- bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
<b> Immunofluorescence staining of fixed MCF7 cells with anti-Fas antibody R-125224 (</b> Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells permewith 0.15% Triton and stained with the chimeric mouse IgG1 version of R-125224 (24-527) at 10 ug/ml for 1h followed by Alexa Fluor®?488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-527- DAPI- merged channels and an isotype control. The isotype control was stained with an anti-unknown specificity antibody (0) followed by Alexa Fluor®?488 secondary antibody.

Documents

Further Information

Additional Names:
CD95, h-HFE7A, Tumor necrosis factor receptor superfamily member 6, CD-95, Apo-1, Apo 1, Apo-1 antigen, Apoptosis-mediating surface antigen FAS, FASLG receptor
Buffer:
PBS with 0.02% Proclin 300.
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
R-125224 is generated by the humanization of the murine HFE7A anti-Fas antibody by grafting the CDR regions to the framework regions of the human 8E10 antibody and substituting key framework residues from the murine antibody into the 8E10 sequence. The original HFE7A was derived from a hybridoma cell line generated by the fusion of NS1 myeloma cells with splenocytes from Fas-deficient mice which had been immunized with partially purified recombinant human Fas-AIC2A chimera protein consisting of the extracellular region of human Fas antigen (aa -16 to 150) and the extracellular region of the murine IL-3 receptor AIC2 (aa 3-423). The HFE7A hybridoma was selected after screening by flow cytometry for the production of antibodies with the ability to bind to the WR19L12a transformed murine T cell lymphoma cell line expressing human Fas or the L5178YA1 cell line expressing murine Fas, but not to the parental WR19L or L5178Y cells.
NCBI Gene ID #:
355
NCBI Official Name:
HGNC:HGNC:11920
NCBI Official Symbol:
FAS
NCBI Organism:
Homo sapiens
Research Area:
Other, Cancer
Swissprot #:
P25445
User NOte:
For research use only.