CBX5 Antibody [RAB-C133], Rabbit IgG

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ProSci
Product Code: PSI-24-836
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-24-836-0.2mg0.2mg£681.00
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Overview

Host Type: Rabbit
Antibody Isotype: IgG kappa
Antibody Clonality: Monoclonal
Antibody Clone: RAB-C133
Regulatory Status: RUO
Target Species: Human
Applications:
  • ChIP
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunoprecipitation-Mass Spectrometry (IP-MS)
Shipping:
Blue Ice
Storage:
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Images

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<b> Western Blot using anti-CBX5 antibody R </b> Nuclear lysates of HeLa(A)- HEK293(B)- A431(C)- MCF7(D)- Jurkat(E) and K562(F) cells (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rsion of R24-836) at 0.003 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
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<b> Immunofluorescence staining of HeLa cells with anti-CBX5 (R/b> Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permewith 0.15% Triton stained with the chimeric r version of R24-836) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution)- showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-836- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.
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<b> Western Blot using anti-CBX5 antibody R </b> Nuclear lysates of HeLa(A)- HEK293(B)- A431(C)- MCF7(D)- Jurkat(E) and K562(F) cells (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rsion of R24-836) at 0.003 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
<b> Immunofluorescence staining of HeLa cells with anti-CBX5 (R/b> Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permewith 0.15% Triton stained with the chimeric r version of R24-836) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution)- showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-836- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.

Documents

Further Information

Additional Names:
p25, Chromobox protein homolog 5, Heterochromatin protein 1 homolog alpha, HP1 alpha, anti-CBX5-RAB-C133, 41D2_35
Buffer:
PBS with 0.02% Proclin 300.
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Human Fab format, for improved compatibility with existing reagents, assays and techniques.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
This antibody was obtained by recombinant antibody (rAb) phage display recognizing CBX5 protein under non-denaturing conditions.
NCBI Gene ID #:
23468
NCBI Official Name:
chromobox 5
NCBI Official Symbol:
CBX5
NCBI Organism:
Homo sapiens
Research Area:
Immunology, Cancer, Cell Cycle
Swissprot #:
P45973
User NOte:
For research use only.