PRDM9 Antibody [RAB-C370], Rabbit IgG

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ProSci
Product Code: PSI-24-850
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-24-850-0.2mg0.2mg£681.00
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Overview

Host Type: Rabbit
Antibody Isotype: IgG kappa
Antibody Clonality: Monoclonal
Antibody Clone: RAB-C370
Regulatory Status: RUO
Target Species: Human
Applications:
  • ChIP
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunoprecipitation-Mass Spectrometry (IP-MS)
Shipping:
Blue Ice
Storage:
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Images

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<b> Immunofluorescence staining of HeLa cells with anti-PRDM9 (R/b> Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permewith 0.15% Triton stained with the chimeric r version of R24-850) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1500 dilution)- showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-850- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.
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<b> Flow cytometry using the Anti-PRDM9 antibody R </b> Paraformaldehyde fixed HeLa cells permewith 0.5% Triton were stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r version of R24-850- blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
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<b> Immunofluorescence staining of HeLa cells with anti-PRDM9 (R/b> Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permewith 0.15% Triton stained with the chimeric r version of R24-850) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1500 dilution)- showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-850- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.
<b> Flow cytometry using the Anti-PRDM9 antibody R </b> Paraformaldehyde fixed HeLa cells permewith 0.5% Triton were stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r version of R24-850- blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Documents

Further Information

Additional Names:
Histone-lysine N-methyltransferase PRDM9, PR domain-containing protein 9, PR domain zinc finger protein 9, Protein-lysine N-methyltransferase PRDM9, [histone H3]-lysine36 N-trimethyltransferase PRDM9, [histone H3]-lysine4 N-trimethyltransferase PRDM9, [histone H3]-lysine9 N-trimethyltransferase PRDM9, [histone H4]-N-methyl-L-lysine20 N-methyltransferase PRDM9, [histone H4]-lysine20 N-methyltransferase PRDM9, EC:2.1.1.-, anti-PRDM9-RAB-C370, PRDM9-A003-1459
Buffer:
PBS with 0.02% Proclin 300.
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Human Fab format, for improved compatibility with existing reagents, assays and techniques.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
This antibody was obtained by recombinant antibody (rAb) phage display recognizing PRDM9 protein under non-denaturing conditions.
NCBI Gene ID #:
56979
NCBI Official Name:
PR/SET domain 9
NCBI Official Symbol:
PRDM9
NCBI Organism:
Homo sapiens
Research Area:
Immunology, Cancer, Cell Cycle
Swissprot #:
Q9NQV7
User NOte:
For research use only.