Ly6G/Ly6C Antibody [RB6-8C5], Rabbit IgG

ProSci
Product Code: PSI-24-595
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-24-595-0.2mg0.2mg£681.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG kappa
Antibody Clonality: Monoclonal
Antibody Clone: RB6-8C5
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Depletion
  • Flow Cytometry
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
Blue Ice
Storage:
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Images

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<b> Immunofluorescence staining of mouse splenocytes using anti-Ly6G/Ly6C antibody (RB6-8C5. </b> Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix™ cover-slips and stained with the chimeric r version of RB6-8C5 (24-595) at 10 ug/ml followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining in subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-595- DAPI- merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (3.0) followed by Alexa Fluor® 488 secondary antibody.
2 / 2

<b> Immunofluorescence staining of mouse splenocytes using anti-Ly6G/Ly6C antibody (RB6-8C5. </b> Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix™ cover-slips and stained with the chimeric r version of RB6-8C5 (24-595) at 10 ug/ml followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining in subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-595- DAPI- merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (3.0) followed by Alexa Fluor® 488 secondary antibody.

Further Information

Additional Names:
Gr-1, Gr1, Ly-6G, ly-6G.1, ly6C
Buffer:
PBS with 0.02% Proclin 300.
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
This antibody was raised in Lou-M rats using a popliteal lymph node immunisation and fusion protocol as described by Martiniello et al (1994)
NCBI Gene ID #:
546644
NCBI Official Name:
lymphocyte antigen 6 complex, locus G
NCBI Official Symbol:
Ly6g
NCBI Organism:
Mus musculus
Research Area:
Immunology, Cancer, Chemokines & Cytokines
Swissprot #:
P35461
User NOte:
For research use only.