CCR5 (PKC site) Antibody [RC-10], Rabbit IgG

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ProSci
Product Code: PSI-24-788
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-24-788-0.2mg0.2mg£681.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG kappa
Antibody Clonality: Monoclonal
Antibody Clone: RC-10
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Shipping:
Blue Ice
Storage:
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Images

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<b> Immunofluorescence staining of fixed U937 cells with anti-CCR5 (PKC site) antibody RC-10 (</b> Immunofluorescence analysis of paraformaldehyde fixed U937 cells on Shi-fix™ coverslips stained with the chimeric r version of RC-10 (24-788) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-788- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (.1) followed by staining with Alexa Fluor® 488 secondary antibody.
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<b> Flow cytometry using the Anti-CCR5 (PKC site) antibody RC-10 ( </b> Paraformaldehyde fixed human peripheral blood monocytes permewith 0.5% Triton were stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r version of RC-10 (24-788- blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
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<b> Immunofluorescence staining of fixed U937 cells with anti-CCR5 (PKC site) antibody RC-10 (</b> Immunofluorescence analysis of paraformaldehyde fixed U937 cells on Shi-fix™ coverslips stained with the chimeric r version of RC-10 (24-788) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-788- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (.1) followed by staining with Alexa Fluor® 488 secondary antibody.
<b> Flow cytometry using the Anti-CCR5 (PKC site) antibody RC-10 ( </b> Paraformaldehyde fixed human peripheral blood monocytes permewith 0.5% Triton were stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r version of RC-10 (24-788- blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Documents

Further Information

Additional Names:
C-C chemokine receptor type 5, C-C CKR-5, CC-CKR-5, CCR-5, CHEMR13, HIV-1 fusion coreceptor, CD195
Buffer:
PBS with 0.02% Proclin 300.
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
This antibody was raised by immunizing mice with CCR5.
NCBI Gene ID #:
1234
NCBI Official Name:
C-C motif chemokine receptor 5
NCBI Official Symbol:
CCR5
NCBI Organism:
Homo sapiens
Research Area:
Immunology, Chemokines & Cytokines
Swissprot #:
P51681
User NOte:
For research use only.