Rhodopsin Antibody [Rho 1D4], Rabbit IgG

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ProSci
Product Code: PSI-24-342
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-24-342-0.2mg0.2mg£681.00
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Overview

Host Type: Rabbit
Antibody Isotype: IgG kappa
Antibody Clonality: Monoclonal
Antibody Clone: Rho 1D4
Regulatory Status: RUO
Target Species:
  • Amphibian
  • Bovine (Cattle)
  • Human
  • Mouse
  • Rat
  • Zebrafish
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Immunoprecipitation (IP)
  • Radioimmune labelling
  • Western Blot (WB)
Shipping:
Blue Ice
Storage:
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Images

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<b> Western Blot using anti-Rhodopsin antibody Rho 1D4 (</b> Rat eye lysate (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rsion of Rho 1D4 (24-342) at 0.0001 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for monomeric rat (<i> Rattus norvegicus </i>) Rhodopsin is 39.0kDa- though this protein in known to form dimers and higher order multimers (c.f. Mansoor et al.- PMID: 16492772- PMID: 10798675). 24-342 successfully detected monomeric- dimeric and multimeric Rhodopsin in rat eye lysate.
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<b> Western Blot using anti-Rhodopsin antibody Rho 1D4 (</b> Rat eye lysate (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rsion of Rho 1D4 (24-342) at 0.0001 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for monomeric rat (<i> Rattus norvegicus </i>) Rhodopsin is 39.0kDa- though this protein in known to form dimers and higher order multimers (c.f. Mansoor et al.- PMID: 16492772- PMID: 10798675). 24-342 successfully detected monomeric- dimeric and multimeric Rhodopsin in rat eye lysate.

Documents

Further Information

Additional Names:
Rho-tag, Visual Purple
Buffer:
PBS with 0.02% Proclin 300.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Bleached bovine rod outer segment (ROS).
NCBI Gene ID #:
6010
NCBI Official Name:
rhodopsin
NCBI Official Symbol:
RHO
NCBI Organism:
Homo sapiens
Research Area:
Other, Neuroscience
Swissprot #:
P08100
User NOte:
For research use only.