CD27 Antibody [RM27-3E5], Rabbit IgG

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ProSci
Product Code: PSI-24-558
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-24-558-0.2mg0.2mg£681.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG kappa
Antibody Clonality: Monoclonal
Antibody Clone: RM27-3E5
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Flow Cytometry
  • Immunoprecipitation (IP)
  • Neutralisation
Shipping:
Blue Ice
Storage:
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Images

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<b>ELISA of anti-CD27 antibody on CD27-Fc fusion protein- with a CD70 (CD27-L) competition assay.</b> Binding curves of the r chimeric version of the anti-CD27 (Interleukin-2 receptor subunit alpha) antibody RM27-3E5 (24-558; red line) and isotype control (anti-Fluorescein antibody 3.0 4-4-20 (enhanced); blue line) to an ELISA plate coated with CD27-Fc fusion protein (Pr00160-1.9) at a concentration of 5 ug/ml. A 3-fold serial dilution from 10 mg/mL to 0.17 ng/ml of 24-558 was used. CD70 (CD27-L) is the ligand for CD27. CD70 Fc-fusion protein (Pr00161-1.28) was added to a 10mg/mL solution of 24-558 starting at 10mg/mL- and then at 3-fold dilutions down to 0.17ng/mL (red line). A negative control assay using 4-1BB Fc-fusion protein (Pr00233-1.9) was also performed (yellow line). Inhibition of the binding of 24-558 to Pr00160-1.9 (CD27) by Pr00161-1.28 (CD70) may be observed between 10-000 ng/mL and 123 ng/mL of Pr00161-23.0- whereas Pr00233-1.9 (4-1BB) does not inhibit 24-558 binding. For signal detection- a 1:4000 dilution of HRP-lnti-human IgG antibody was used.
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<b>ELISA of anti-CD27 antibody on CD27-Fc fusion protein- with a CD70 (CD27-L) competition assay.</b> Binding curves of the r chimeric version of the anti-CD27 (Interleukin-2 receptor subunit alpha) antibody RM27-3E5 (24-558; red line) and isotype control (anti-Fluorescein antibody 3.0 4-4-20 (enhanced); blue line) to an ELISA plate coated with CD27-Fc fusion protein (Pr00160-1.9) at a concentration of 5 ug/ml. A 3-fold serial dilution from 10 mg/mL to 0.17 ng/ml of 24-558 was used. CD70 (CD27-L) is the ligand for CD27. CD70 Fc-fusion protein (Pr00161-1.28) was added to a 10mg/mL solution of 24-558 starting at 10mg/mL- and then at 3-fold dilutions down to 0.17ng/mL (red line). A negative control assay using 4-1BB Fc-fusion protein (Pr00233-1.9) was also performed (yellow line). Inhibition of the binding of 24-558 to Pr00160-1.9 (CD27) by Pr00161-1.28 (CD70) may be observed between 10-000 ng/mL and 123 ng/mL of Pr00161-23.0- whereas Pr00233-1.9 (4-1BB) does not inhibit 24-558 binding. For signal detection- a 1:4000 dilution of HRP-lnti-human IgG antibody was used.

Documents

Further Information

Additional Names:
TNFRS7, CD27 antigen, CD27L receptor, T-cell activation antigen CD27, Tumor necrosis factor receptor superfamily member 7
Buffer:
PBS with 0.02% Proclin 300.
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
This antibody was raised by immunising mouse with mouse CD27-human IgG1 Fc fusion protein. Isolated popliteal lymph node cells were then fused with P3U1 myeloma cells to produce stable hybridomas.
NCBI Gene ID #:
21940
NCBI Official Name:
CD27 antigen
NCBI Official Symbol:
Cd27
NCBI Organism:
Mus musculus
Research Area:
Immunology, Neuroscience
Swissprot #:
P41272
User NOte:
For research use only.