IL-9 Antibody [RM9A4], Rabbit IgG

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ProSci
Product Code: PSI-24-673
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-24-673-0.2mg0.2mg£681.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG kappa
Antibody Clonality: Monoclonal
Antibody Clone: RM9A4
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Flow Cytometry
Shipping:
Blue Ice
Storage:
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Images

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<b> Flow-cytometry using the Anti-IL-9 antibody RM9A4 ( </b> Mouse splenocytes were stimulated with PMA and Ionomycin in presence of Monensin for 4 hours. The cells were fixed using 2% PFA- permeusing 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control- black line) or the r version of RM9A4 (24-673- pink line) at a dilution of 1:100 for 1h at RT. The blue line shows unstimulated human peripheral blood leukocytes stained with 24-673. After washing- bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.
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<b> Flow-cytometry using the Anti-IL-9 antibody RM9A4 ( </b> Mouse splenocytes were stimulated with PMA and Ionomycin in presence of Monensin for 4 hours. The cells were fixed using 2% PFA- permeusing 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control- black line) or the r version of RM9A4 (24-673- pink line) at a dilution of 1:100 for 1h at RT. The blue line shows unstimulated human peripheral blood leukocytes stained with 24-673. After washing- bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.

Documents

Further Information

Additional Names:
Interleukin-9, IL9, Cytokine P40, T-cell growth factor P40
Buffer:
PBS with 0.02% Proclin 300.
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
This antibody was raised by immunising rats with recombinant full-length murine IL-9.
NCBI Gene ID #:
16198
NCBI Official Name:
interleukin 9
NCBI Official Symbol:
Il9
NCBI Organism:
Mus musculus
Research Area:
Chemokines & Cytokines, Immunology
Swissprot #:
P15247
User NOte:
For research use only.