TIM-2 Antibody [RMT2-14], Rabbit IgG

ProSci
Product Code: PSI-24-629
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-24-629-0.2mg0.2mg£681.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG lambda
Antibody Clonality: Monoclonal
Antibody Clone: RMT2-14
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Blocking
  • Flow Cytometry
  • Functional Study
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
Blue Ice
Storage:
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Images

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<b> Immunofluorescence staining of fixed mouse splenocytes with anti-TIM-2 antibody RMT2-14 (</b> Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix™ coverslips stained with the chimeric r version of RMT2-14 (24-629) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-629- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (.1) followed by staining with Alexa Fluor® 488 secondary antibody.

<b> Immunofluorescence staining of fixed mouse splenocytes with anti-TIM-2 antibody RMT2-14 (</b> Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix™ coverslips stained with the chimeric r version of RMT2-14 (24-629) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-629- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (.1) followed by staining with Alexa Fluor® 488 secondary antibody.

Further Information

Additional Names:
TIMD2, T Cell Immunoglobulin Mucin-2, T Cell immunoglobulin and mucin domain protein 2, TIM3
Buffer:
PBS with 0.02% Proclin 300.
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
This antibody was raised by immunising Sprague Dawley rats with TIM-2-Ig consisting of the extracellular domain (aa 1-230 of mouse TIM-2) and the Fc portion of mouse IgG2a, emulsified in complete Freund's adjuvant and then fusing lymph node cells from the immunized rats with P3U1 myeloma cells.
NCBI Gene ID #:
171284
NCBI Official Name:
T cell immunoglobulin and mucin domain containing 2
NCBI Official Symbol:
Timd2
NCBI Organism:
Mus musculus
Research Area:
Immunology, Cell Cycle, Neuroscience
Swissprot #:
Q8R183
User NOte:
For research use only.