Beta-Tubulin Antibody [S11B], Rabbit IgG

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ProSci
Product Code: PSI-24-367
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-24-367-0.2mg0.2mg£681.00
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Overview

Host Type: Rabbit
Antibody Isotype: IgG lambda
Antibody Clonality: Monoclonal
Antibody Clone: S11B
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Shipping:
Blue Ice
Storage:
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Images

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<b> Immunofluoresence staining of fixed HeLa cells with anti-Beta-tubulin antibody S11B (</b> Immunofluorescence analysis of paraformaldehyde fixed HeLa cells- permewith 0.15% Triton stained with the chimeric r version of S11B (24-367) at 10 ug/ml for 1h followed by Alexa Fluor®?488 secondary antibody (1 ug/ml)- showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-367- DAPI- merged channels and a negative control. The negative control was stained with unimmunized r followed by Alexa Fluor®?488 secondary antibody.
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<b> Western Blot using anti-Beta-Tubulin antibody S11B (</b> HeLa (A)- A431 (B)- HEK293 (C) and MCF-7 (D) cell lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rsion of S11B (24-367) at 0.01 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for Beta-Tubulin is ~54kDa. 24-367 successfully detected human Beta-Tubulin in HeLa- A431- HEK293 and MCF-7 cell lysate samples.
4 / 4
<b> Immunohistochemical staining of human cerebral cortex tissue using anti-Beta Tubulin antibody (S11B </b> Anti-Beta Tubulin staining of paraffin embedded human cerebral cortex tissue using the rmeric version of S11B (24-367). Antigen retreival was acheived by microwaving in citrate buffer (pH6)- followed by blocking with protein block serum-free buffer (Dako- cat. #X0909). Primary antibody incubation with 24-367 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-r HRP secondary antibody (Dako cat#K4009) for 20 mins followed by Ddiaminobenzidine)- and counter-staining with haemotoxylin. Staining of neuronal cell bodies and their processes may be observed. Recommended concentration- 2-4 ug/ml.
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<b> Immunofluoresence staining of fixed HeLa cells with anti-Beta-tubulin antibody S11B (</b> Immunofluorescence analysis of paraformaldehyde fixed HeLa cells- permewith 0.15% Triton stained with the chimeric r version of S11B (24-367) at 10 ug/ml for 1h followed by Alexa Fluor®?488 secondary antibody (1 ug/ml)- showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-367- DAPI- merged channels and a negative control. The negative control was stained with unimmunized r followed by Alexa Fluor®?488 secondary antibody.
<b> Western Blot using anti-Beta-Tubulin antibody S11B (</b> HeLa (A)- A431 (B)- HEK293 (C) and MCF-7 (D) cell lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rsion of S11B (24-367) at 0.01 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for Beta-Tubulin is ~54kDa. 24-367 successfully detected human Beta-Tubulin in HeLa- A431- HEK293 and MCF-7 cell lysate samples.
<b> Immunohistochemical staining of human cerebral cortex tissue using anti-Beta Tubulin antibody (S11B </b> Anti-Beta Tubulin staining of paraffin embedded human cerebral cortex tissue using the rmeric version of S11B (24-367). Antigen retreival was acheived by microwaving in citrate buffer (pH6)- followed by blocking with protein block serum-free buffer (Dako- cat. #X0909). Primary antibody incubation with 24-367 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-r HRP secondary antibody (Dako cat#K4009) for 20 mins followed by Ddiaminobenzidine)- and counter-staining with haemotoxylin. Staining of neuronal cell bodies and their processes may be observed. Recommended concentration- 2-4 ug/ml.

Documents

Further Information

Additional Names:
TUBB
Buffer:
PBS with 0.02% Proclin 300.
Chimeric Use Statement:
This full-length, chimeric rabbit antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Human MBP (microtubule-binding protein).
NCBI Gene ID #:
10381
NCBI Official Name:
tubulin beta 3 class III
NCBI Official Symbol:
TUBB3
NCBI Organism:
Homo sapiens
Research Area:
Cell Cycle, Cancer, Other
Swissprot #:
Q13509
User NOte:
For research use only.