XPNPEP1 Antibody [SAIC-07E-1], Rabbit IgG

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ProSci
Product Code: PSI-24-309
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-24-309-0.2mg0.2mg£681.00
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Overview

Host Type: Rabbit
Antibody Isotype: IgG kappa
Antibody Clonality: Monoclonal
Antibody Clone: SAIC-07E-1
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immuno-MRM
  • Western Blot (WB)
Shipping:
Blue Ice
Storage:
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Images

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<b> Immunofluorescence staining of HeLa cells with anti-XPNPEP1 (SAIC-07E-1 </b> Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permewith 0.15% Triton stained with the chimeric r version of SAIC-07E-1 (24-309) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution)- showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-309- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.
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<b> Western Blot using anti-XPNPEP1 antibody SAIC-07E-1 ( </b> HeLa(A) (0.003ug/ml)- Jurkat(B) (0.001ug/ml)- MCF7(C) (0.001ug/ml)- HepG2(D) (0.003ug/ml)- A549(E) (0.001ug/ml) and HEK293(F) (0.003ug/ml) cell lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rsion of SAIC-07E-1 (24-309)- before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
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<b> Immunofluorescence staining of HeLa cells with anti-XPNPEP1 (SAIC-07E-1 </b> Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permewith 0.15% Triton stained with the chimeric r version of SAIC-07E-1 (24-309) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution)- showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-309- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.
<b> Western Blot using anti-XPNPEP1 antibody SAIC-07E-1 ( </b> HeLa(A) (0.003ug/ml)- Jurkat(B) (0.001ug/ml)- MCF7(C) (0.001ug/ml)- HepG2(D) (0.003ug/ml)- A549(E) (0.001ug/ml) and HEK293(F) (0.003ug/ml) cell lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rsion of SAIC-07E-1 (24-309)- before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Documents

Further Information

Additional Names:
Xaa-Pro aminopeptidase 1, XPNPEPL, XPNPEPL1
Buffer:
PBS with 0.02% Proclin 300.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Peptide "TLSLDEVYLIDSGAQYK" derived from Xaa-Pro aminopeptidase 1 conjugated to KLH.
NCBI Gene ID #:
7511
NCBI Official Name:
X-prolyl aminopeptidase 1
NCBI Official Symbol:
XPNPEP1
NCBI Organism:
Homo sapiens
Research Area:
Immunology
Swissprot #:
Q9NQW7
User NOte:
For research use only.