ANXA4 Antibody [SAIC-14C-10F12], Rabbit IgG

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ProSci
Product Code: PSI-24-311
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-24-311-0.2mg0.2mg£681.00
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Overview

Host Type: Rabbit
Antibody Isotype: IgG kappa
Antibody Clonality: Monoclonal
Antibody Clone: SAIC-14C-10F12
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immuno-MRM
  • Western Blot (WB)
Shipping:
Blue Ice
Storage:
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Images

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<b> Immunofluorescence staining of HepG2 cells with anti-ANXA4 (SAIC-14C-10F12 </b> Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells permewith 0.15% Triton stained with the chimeric r version of SAIC-14C-10F12 (24-311) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution)- showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-311- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.
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<b> Flow cytometry using the Anti-ANXA4 antibody SAIC-14C-10F12 ( </b> Paraformaldehyde fixed HepG2 cells permewith 0.5% Triton were stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r version of SAIC-14C-10F12 (24-311- blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
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<b> Immunofluorescence staining of HepG2 cells with anti-ANXA4 (SAIC-14C-10F12 </b> Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells permewith 0.15% Triton stained with the chimeric r version of SAIC-14C-10F12 (24-311) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution)- showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-311- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.
<b> Flow cytometry using the Anti-ANXA4 antibody SAIC-14C-10F12 ( </b> Paraformaldehyde fixed HepG2 cells permewith 0.5% Triton were stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r version of SAIC-14C-10F12 (24-311- blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Documents

Further Information

Additional Names:
ANXA4 protein
Buffer:
PBS with 0.02% Proclin 300.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Peptide "DEGNYLDDALVR" derived from Annexin A4 conjugated to KLH.
NCBI Gene ID #:
307
NCBI Official Name:
annexin A4
NCBI Official Symbol:
ANXA4
NCBI Organism:
Homo sapiens
Research Area:
Immunology
Swissprot #:
P09525
User NOte:
For research use only.