TAGLN Antibody [SAIC-33B-129], Rabbit IgG

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ProSci
Product Code: PSI-24-331
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-24-331-0.2mg0.2mg£681.00
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Overview

Host Type: Rabbit
Antibody Isotype: IgG kappa
Antibody Clonality: Monoclonal
Antibody Clone: SAIC-33B-129
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immuno-MRM
  • Western Blot (WB)
Shipping:
Blue Ice
Storage:
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Images

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<b> Immunofluorescence staining of HeLa cells with anti-TAGLN (SAIC-33B-129 </b> Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permewith 0.15% Triton stained with the chimeric r version of SAIC-33B-129 (24-331) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution)- showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-331- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.
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<b> Western Blot using anti-TAGLN antibody SAIC-33B-129 ( </b> HeLa(A)- Caco-2(B)- U2OS(C) cell lysate and human placenta(D)- muscle(E) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rsion of SAIC-33B-129 (24-331) at 0.0003 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
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<b> Immunofluorescence staining of HeLa cells with anti-TAGLN (SAIC-33B-129 </b> Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permewith 0.15% Triton stained with the chimeric r version of SAIC-33B-129 (24-331) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution)- showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-331- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.
<b> Western Blot using anti-TAGLN antibody SAIC-33B-129 ( </b> HeLa(A)- Caco-2(B)- U2OS(C) cell lysate and human placenta(D)- muscle(E) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rsion of SAIC-33B-129 (24-331) at 0.0003 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Documents

Further Information

Additional Names:
Transgelin,SM22,WS3-10
Buffer:
PBS with 0.02% Proclin 300.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Peptide "AAEDYGVIK", derived from the actin cross-linking protein Transgelin, conjugated to KLH.
NCBI Gene ID #:
6876
NCBI Official Name:
transgelin
NCBI Official Symbol:
TAGLN
NCBI Organism:
Homo sapiens
Research Area:
Immunology
Swissprot #:
Q01995
User NOte:
For research use only.