UBE2C Antibody [SAIC-41A-1], Rabbit IgG

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ProSci
Product Code: PSI-24-338
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-24-338-0.2mg0.2mg£681.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG kappa
Antibody Clonality: Monoclonal
Antibody Clone: SAIC-41A-1
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immuno-MRM
  • Western Blot (WB)
Shipping:
Blue Ice
Storage:
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Images

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<b> Immunofluorescence staining of Caco-2 cells with anti-UBE2C (SAIC-41A-1 </b> Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells permewith 0.15% Triton stained with the chimeric r version of SAIC-41A-1 (24-338) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution)- showing NUCLEAR staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-338- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.
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<b> Flow cytometry using the anti-UBE2C antibody SAIC-41A-1 ( </b> Paraformaldehyde fixed MCF7 cells permewith 0.5% Triton were stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r version of SAIC-41A-1 (24-338- blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
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<b> Immunofluorescence staining of Caco-2 cells with anti-UBE2C (SAIC-41A-1 </b> Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells permewith 0.15% Triton stained with the chimeric r version of SAIC-41A-1 (24-338) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution)- showing NUCLEAR staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-338- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.
<b> Flow cytometry using the anti-UBE2C antibody SAIC-41A-1 ( </b> Paraformaldehyde fixed MCF7 cells permewith 0.5% Triton were stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r version of SAIC-41A-1 (24-338- blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Documents

Further Information

Additional Names:
Ubiquitin-conjugating enzyme E2 C, UBCH10
Buffer:
PBS with 0.02% Proclin 300.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Peptide "GISAFPESDNLFK", derived from the Ubiquitin-conjugating enzyme E2C, conjugated to KLH.
NCBI Gene ID #:
11065
NCBI Official Name:
ubiquitin conjugating enzyme E2 C
NCBI Official Symbol:
UBE2C
NCBI Organism:
Homo sapiens
Research Area:
Immunology
Swissprot #:
O00762
User NOte:
For research use only.