CLU Antibody [SAIC-43B-8], Rabbit IgG

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ProSci
Product Code: PSI-24-340
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-24-340-0.2mg0.2mg£681.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG kappa
Antibody Clonality: Monoclonal
Antibody Clone: SAIC-43B-8
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immuno-MRM
  • Western Blot (WB)
Shipping:
Blue Ice
Storage:
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Images

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<b> Flow cytometry using the Anti-CLU antibody SAIC-43B-8 ( </b> Paraformaldehyde fixed HeLa cells permewith 0.5% Triton were stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r version of SAIC-43B-8 (24-340- blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
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<b> Immunofluorescence staining of HeLa cells with anti-CLU (SAIC-43B-8 </b> Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permewith 0.15% Triton stained with the chimeric r version of SAIC-43B-8 (24-340) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution)- showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-340- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.
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<b> Flow cytometry using the Anti-CLU antibody SAIC-43B-8 ( </b> Paraformaldehyde fixed HeLa cells permewith 0.5% Triton were stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r version of SAIC-43B-8 (24-340- blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
<b> Immunofluorescence staining of HeLa cells with anti-CLU (SAIC-43B-8 </b> Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permewith 0.15% Triton stained with the chimeric r version of SAIC-43B-8 (24-340) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution)- showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-340- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.

Documents

Further Information

Additional Names:
Clusterin, APOJ, CLI, KUB1, AAG4
Buffer:
PBS with 0.02% Proclin 300.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Peptide "ASSIIDELFQDR" derived from Clusterin conjugated to KLH. Clusterin isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins
NCBI Gene ID #:
1191
NCBI Official Name:
clusterin
NCBI Official Symbol:
CLU
NCBI Organism:
Homo sapiens
Research Area:
Immunology
Swissprot #:
P10909
User NOte:
For research use only.