IL-8RA Antibody [SE2], Rabbit IgG

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ProSci
Product Code: PSI-24-791
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-24-791-0.2mg0.2mg£681.00
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Overview

Host Type: Rabbit
Antibody Isotype: IgG kappa
Antibody Clonality: Monoclonal
Antibody Clone: SE2
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
Blue Ice
Storage:
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Images

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<b> Immunofluorescence staining of fixed human peripheral blood leukocytes with anti-IL-8RA antibody SE2 (</b> Immunofluorescence analysis of paraformaldehyde fixed human peripheral blood leukocytes on Shi-fix™ coverslips stained with the chimeric r version of SE2 (24-791) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-791- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (.1) followed by staining with Alexa Fluor® 488 secondary antibody.
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<b> Flow cytometry using the Anti-IL-8RA antibody SE2 ( </b> Paraformaldehyde fixed human peripheral blood monocytes were stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r version of SE2 (24-791- blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
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<b> Immunofluorescence staining of fixed human peripheral blood leukocytes with anti-IL-8RA antibody SE2 (</b> Immunofluorescence analysis of paraformaldehyde fixed human peripheral blood leukocytes on Shi-fix™ coverslips stained with the chimeric r version of SE2 (24-791) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-791- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (.1) followed by staining with Alexa Fluor® 488 secondary antibody.
<b> Flow cytometry using the Anti-IL-8RA antibody SE2 ( </b> Paraformaldehyde fixed human peripheral blood monocytes were stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r version of SE2 (24-791- blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Documents

Further Information

Additional Names:
CXCR-1, C-X-C chemokine receptor type 1, CXC-R1, CDw128a, High affinity interleukin-8 receptor A, IL-8R A, IL-8 receptor type 1, CD181, interleukin 8
Buffer:
PBS with 0.02% Proclin 300.
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
This antibody was raised by immunizing BALB/c mice with a BSA-conjugated peptide comprising of the N-terminal part of IL-8RA (amino acids 1-30).
NCBI Gene ID #:
3577
NCBI Official Name:
C-X-C motif chemokine receptor 1
NCBI Official Symbol:
CXCR1
NCBI Organism:
Homo sapiens
Research Area:
Neuroscience, Chemokines & Cytokines, Immunology, Other
Swissprot #:
P25024
User NOte:
For research use only.