HER2/neu Antibody [SER4], Mouse IgG1

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ProSci
Product Code: PSI-24-356
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-24-356-0.2mg0.2mg£681.00
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Overview

Host Type: Mouse
Antibody Isotype: IgG1 kappa
Antibody Clonality: Monoclonal
Antibody Clone: SER4
Regulatory Status: RUO
Target Species: Human
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
Blue Ice
Storage:
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Images

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<b> Immunofluorescence staining of MCF7 cells using anti-HER2/neu antibody (SER4. </b> Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells stained with the chimeric mouse IgG version of SER4 (24-356) at 10 ug/ml followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-356- DAPI- merged channels and an isotype control. The isotype control was stained with anti-unknown antibody (.1) followed by Alexa Fluor® 488 secondary antibody.
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<b> Flow cytometry using the anti-HER2/neu antibody SER4 ( </b> Paraformaldehyde fixed MCF7 cells were stained with anti-unknown specificity antibody (.1; isotype control- black line) or the mouse IgG1 version of SER4 (24-356- blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
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<b> Immunofluorescence staining of MCF7 cells using anti-HER2/neu antibody (SER4. </b> Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells stained with the chimeric mouse IgG version of SER4 (24-356) at 10 ug/ml followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-356- DAPI- merged channels and an isotype control. The isotype control was stained with anti-unknown antibody (.1) followed by Alexa Fluor® 488 secondary antibody.
<b> Flow cytometry using the anti-HER2/neu antibody SER4 ( </b> Paraformaldehyde fixed MCF7 cells were stained with anti-unknown specificity antibody (.1; isotype control- black line) or the mouse IgG1 version of SER4 (24-356- blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Documents

Further Information

Additional Names:
ERBB2, Receptor tyrosine-protein kinase erbB-2, human epidermal growth factor receptor 2,
Buffer:
PBS with 0.02% Proclin 300.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Recombinant HER2/neu.
NCBI Gene ID #:
2064
NCBI Official Name:
erb-b2 receptor tyrosine kinase 2
NCBI Official Symbol:
ERBB2
NCBI Organism:
Homo sapiens
Research Area:
Cancer, Cell Cycle
Swissprot #:
P04626
User NOte:
For research use only.