MUC1 Antibody [SM3], Rabbit IgG

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ProSci
Product Code: PSI-24-248
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-24-248-0.2mg0.2mg£681.00
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Overview

Host Type: Rabbit
Antibody Isotype: IgG lambda
Antibody Clonality: Monoclonal
Antibody Clone: SM3
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Flow Cytometry
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Shipping:
Blue Ice
Storage:
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Images

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<b> Western Blot using anti-MUC1 antibody SM3 (</b> MCF-7 cell lysate (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rsion of SM3 (24-248) at 1 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The predicted band size for unmodified MUC1 is 122.1kDa- though in breast cancer cell lines like MCF-7 MUC1 can be up to 90% glycosylated (c.f. Mueller et al. PMID: 10373415; T47D cells) and expected band sizes are ~250-300kDa. Thus the two bands likely represent processed (>250kDa) and unprocessed (~121kDa) populations of the protein. 24-248 successfully detected human MUC1 in MCF-7 breast cancer cells.
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3 / 4
<b> Immunofluorescence staining of fixed MCF-7 cells with anti-MUC1 antibody SM3 (</b> Immunofluorescence analysis of unpermearaformaldehyde fixed MCF-7 cells on Shi-fix™ coverslips stained with the chimeric rsion of SM3 (24-248) at 10 ug/ml for 1h followed by Alexa Fluor®?488 secondary antibody (1 ug/ml)- showing clear membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom; 24-248- DAPI- merged channels and an isotype control. The isotype control was stained with unimmunised r followed by Alexa Fluor®?488 secondary antibody.
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<b> Immunohistochemical staining of human lung tissue using anti-MUC1 antibody (SM3 </b> Anti-MUC1 (Mucin-1) staining of paraffin embedded human lung tissue using the rmeric version of SM3 (24-248). Antigen retreival was acheived by microwaving in citrate buffer (pH6)- followed by blocking with protein block serum-free buffer (Dako- cat. #X0909). Primary antibody incubation with 24-248 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-r HRP secondary antibody (Dako cat#K4009) for 20 mins followed by Ddiaminobenzidine)- and counter-staining with haemotoxylin. Staining of type II pneumocytes may be observed. Recommended concentration- 2-4 ug/ml.
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<b> Western Blot using anti-MUC1 antibody SM3 (</b> MCF-7 cell lysate (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rsion of SM3 (24-248) at 1 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The predicted band size for unmodified MUC1 is 122.1kDa- though in breast cancer cell lines like MCF-7 MUC1 can be up to 90% glycosylated (c.f. Mueller et al. PMID: 10373415; T47D cells) and expected band sizes are ~250-300kDa. Thus the two bands likely represent processed (>250kDa) and unprocessed (~121kDa) populations of the protein. 24-248 successfully detected human MUC1 in MCF-7 breast cancer cells.
<b> Immunofluorescence staining of fixed MCF-7 cells with anti-MUC1 antibody SM3 (</b> Immunofluorescence analysis of unpermearaformaldehyde fixed MCF-7 cells on Shi-fix™ coverslips stained with the chimeric rsion of SM3 (24-248) at 10 ug/ml for 1h followed by Alexa Fluor®?488 secondary antibody (1 ug/ml)- showing clear membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom; 24-248- DAPI- merged channels and an isotype control. The isotype control was stained with unimmunised r followed by Alexa Fluor®?488 secondary antibody.
<b> Immunohistochemical staining of human lung tissue using anti-MUC1 antibody (SM3 </b> Anti-MUC1 (Mucin-1) staining of paraffin embedded human lung tissue using the rmeric version of SM3 (24-248). Antigen retreival was acheived by microwaving in citrate buffer (pH6)- followed by blocking with protein block serum-free buffer (Dako- cat. #X0909). Primary antibody incubation with 24-248 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-r HRP secondary antibody (Dako cat#K4009) for 20 mins followed by Ddiaminobenzidine)- and counter-staining with haemotoxylin. Staining of type II pneumocytes may be observed. Recommended concentration- 2-4 ug/ml.

Documents

Further Information

Additional Names:
Mucin
Buffer:
PBS with 0.02% Proclin 300.
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original murine IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Hydrogen fluoride deglycosylated milk mucin.
NCBI Gene ID #:
4582
NCBI Official Name:
HGNC:HGNC:7508
NCBI Official Symbol:
MUC1
NCBI Organism:
Homo sapiens
Research Area:
Cancer, Immunology, Other
Swissprot #:
P15941
User NOte:
For research use only.