TRP-1, gp75 Antibody [TA99], Rabbit IgG

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ProSci
Product Code: PSI-24-232
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-24-232-0.2mg0.2mg£681.00
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Overview

Host Type: Rabbit
Antibody Isotype: IgG kappa
Antibody Clonality: Monoclonal
Antibody Clone: TA99
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Flow Cytometry
  • Immunofluorescence (IF)
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Shipping:
Blue Ice
Storage:
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Images

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<b> Flow-cytometry using anti-CD3 epsilon (2C11 scFv) and TRP1 (TA99) antibodies. </b> Mouse splenocytes (A)- B16F10 murine melanoma cells (B)- KPC3 pacreas carcinoma cells (C) and KPC3 cells transfected with the Trp1 gene (D) were fixed using 2% PFA- permeusing 0.5% Triton and were subject to a primary treatment of either buffer- mouse-IgG1 chimeric 2C11 or mouse-IgG1 chimeric TA99 (indicated ts) before a secondary treatment with buffer- goat anti-mouse Ig-allophycocyanin (G-aM Ig-APC) or anti-HisTag-APC (aHis-APC) antibodies (indicated beside plots). In panel A- splenocytes were also stained with a commercially avail-CD3 (2C11) antibody conjugated to phycoerythrin (PE); all cells (i-v) were CD3 and thus PE positive. In subpanel 'A v' an increase in APC fluorescence intensity (FI(APC)) indicates binding of aHis-APC to 2C11 bound to CD3 at the cell surface. Some Ig containing proteins expressed by the splenocytes may explain the increase in APC fluorescence in subpanel 'A iii'. In panel B an increase in FI(APC) in subpanel 'iii' indicates that TA99 binds to heavily expressed TRP1 at B16F10 cell surfaces and is then detectg an G-aM Ig-APC antibody. Conversely- G-aM Ig-APC did not detect 2C11 at the cell surface- whereas a subset of cells with 2C11 bound to the surface were detectg aHis-APC. Panel C shows that TRP1 is not detectPC3 carcinoma cells ('Ci- iii- v') as expected- and that again- aHis-APC is etect a small subset of CD3 expressing cells ('C vi'). When transfected with the Trp1 gene- KPC3 cells then strongly express TRP1 and it becomes detectiii'). A small subset of CD3 positive cells was again detectrp1 transfected KPC3 cells ('D vi'). All analyses were made using FACSCanto flow-cytometer.
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<b> Flow-cytometry using anti-CD3 epsilon (2C11 scFv) and TRP1 (TA99) antibodies. </b> Mouse splenocytes (A)- B16F10 murine melanoma cells (B)- KPC3 pacreas carcinoma cells (C) and KPC3 cells transfected with the Trp1 gene (D) were fixed using 2% PFA- permeusing 0.5% Triton and were subject to a primary treatment of either buffer- mouse-IgG1 chimeric 2C11 or mouse-IgG1 chimeric TA99 (indicated ts) before a secondary treatment with buffer- goat anti-mouse Ig-allophycocyanin (G-aM Ig-APC) or anti-HisTag-APC (aHis-APC) antibodies (indicated beside plots). In panel A- splenocytes were also stained with a commercially avail-CD3 (2C11) antibody conjugated to phycoerythrin (PE); all cells (i-v) were CD3 and thus PE positive. In subpanel 'A v' an increase in APC fluorescence intensity (FI(APC)) indicates binding of aHis-APC to 2C11 bound to CD3 at the cell surface. Some Ig containing proteins expressed by the splenocytes may explain the increase in APC fluorescence in subpanel 'A iii'. In panel B an increase in FI(APC) in subpanel 'iii' indicates that TA99 binds to heavily expressed TRP1 at B16F10 cell surfaces and is then detectg an G-aM Ig-APC antibody. Conversely- G-aM Ig-APC did not detect 2C11 at the cell surface- whereas a subset of cells with 2C11 bound to the surface were detectg aHis-APC. Panel C shows that TRP1 is not detectPC3 carcinoma cells ('Ci- iii- v') as expected- and that again- aHis-APC is etect a small subset of CD3 expressing cells ('C vi'). When transfected with the Trp1 gene- KPC3 cells then strongly express TRP1 and it becomes detectiii'). A small subset of CD3 positive cells was again detectrp1 transfected KPC3 cells ('D vi'). All analyses were made using FACSCanto flow-cytometer.

Documents

Further Information

Additional Names:
Melanoma antigen gp75, 5,6-dihydroxyindole-2-carboxylic acid oxidase, DHICA oxidase, Catalase B, Glycoprotein 75, Tyrosinase-related protein 1, TRP, TRP-1, TRP1
Buffer:
PBS with 0.02% Proclin 300.
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
70-75 kDa pigmentation-associated glycoprotein in human melanoma cell lines.
NCBI Gene ID #:
7306
NCBI Official Name:
tyrosinase related protein 1
NCBI Official Symbol:
TYRP1
NCBI Organism:
Homo sapiens
Research Area:
Cancer, Other
Swissprot #:
P17643
User NOte:
For research use only.