CCR5 (phosphoserine 337) Antibody [V14/2], Rabbit IgG

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ProSci
Product Code: PSI-24-785
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-24-785-0.2mg0.2mg£681.00
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Overview

Host Type: Rabbit
Antibody Isotype: IgG kappa
Antibody Clonality: Monoclonal
Antibody Clone: V14/2
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Flow Cytometry
  • Immunofluorescence (IF)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Shipping:
Blue Ice
Storage:
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Images

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<b> Flow cytometry using the anti-CCR5 (phosphoserine 349) antibody E11/19 ( </b> U937 cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r1 version of E11/19 (24-785- blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
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<b> Western Blot using anti-CCR5 (phosphoserine 337) antibody V14/2 (</b> K562 cell lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rsion of V14/2 (24-785) at 2ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
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<b> Flow cytometry using the anti-CCR5 (phosphoserine 349) antibody E11/19 ( </b> U937 cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r1 version of E11/19 (24-785- blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
<b> Western Blot using anti-CCR5 (phosphoserine 337) antibody V14/2 (</b> K562 cell lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rsion of V14/2 (24-785) at 2ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Documents

Further Information

Additional Names:
CD195, C-C chemokine receptor type 5, C-C CKR-5, CC-CKR-5, CCR-5, CHEMR13, HIV-1 fusion coreceptor, p337, pS337, Ser-337, Ser337
Buffer:
PBS with 0.02% Proclin 300.
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
This antibody was raised by immunizing mice with a synthetic phospho peptide corresponding to the C-Terminal region of human CCR5 conjugated to BSA.
NCBI Gene ID #:
1234
NCBI Official Name:
C-C motif chemokine receptor 5
NCBI Official Symbol:
CCR5
NCBI Organism:
Homo sapiens
Research Area:
Immunology, Chemokines & Cytokines, Other
Swissprot #:
P51681
User NOte:
For research use only.