Amyloid beta (aa 4-10) Antibody [WO2], Mouse IgG2a

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ProSci
Product Code: PSI-24-267
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-24-267-0.1mg0.1mg£850.00
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Overview

Host Type: Mouse
Antibody Isotype: IgG2a kappa
Antibody Clonality: Monoclonal
Antibody Clone: WO2
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Shipping:
Blue Ice
Storage:
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Images

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<b> Comparison of recombinant WO2 anti-Amyloid beta (24-267) with hybridoma-derived WO2 from Competitor M. </b> Panel <b> A</b> shows a Coomassie Blue-stained SDS-PAGE gel comparing Antibody?s recombinant WO2 with antibody from the corresponding hybridoma under native and reducing (DTT) conditions. Both preparations showed- by this method- comparative purity. Subsequently- the specificity of the antibodies was tested in assays detecting immobilised peptides (see <b>B</b>)- in particular various Amyloid-beta derived peptides probed with WO2 (50 ng/ml) and a goat-anti-mouse peroxidase conjugated tracer as shown in <b>C</b>. The recombinant and hybridoma-derived antibody showed comparative selectivity- however higher signal intensity. Titration of recombinant and hybridoma derived WO2 against A? 2-42 (<b>D</b>)and A? 3-42(<b>E</b>) indicates that the recombinant antibody has higher activity than the hybridoma-derived counterpart.
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<b> Comparison of recombinant WO2 anti-Amyloid beta (24-267) with hybridoma-derived WO2 from Competitor M. </b> Panel <b> A</b> shows a Coomassie Blue-stained SDS-PAGE gel comparing Antibody?s recombinant WO2 with antibody from the corresponding hybridoma under native and reducing (DTT) conditions. Both preparations showed- by this method- comparative purity. Subsequently- the specificity of the antibodies was tested in assays detecting immobilised peptides (see <b>B</b>)- in particular various Amyloid-beta derived peptides probed with WO2 (50 ng/ml) and a goat-anti-mouse peroxidase conjugated tracer as shown in <b>C</b>. The recombinant and hybridoma-derived antibody showed comparative selectivity- however higher signal intensity. Titration of recombinant and hybridoma derived WO2 against A? 2-42 (<b>D</b>)and A? 3-42(<b>E</b>) indicates that the recombinant antibody has higher activity than the hybridoma-derived counterpart.
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<b> Comparison of recombinant WO2 anti-Amyloid beta (24-267) with hybridoma-derived WO2 from Competitor M. </b> Panel <b> A</b> shows a Coomassie Blue-stained SDS-PAGE gel comparing Antibody?s recombinant WO2 with antibody from the corresponding hybridoma under native and reducing (DTT) conditions. Both preparations showed- by this method- comparative purity. Subsequently- the specificity of the antibodies was tested in assays detecting immobilised peptides (see <b>B</b>)- in particular various Amyloid-beta derived peptides probed with WO2 (50 ng/ml) and a goat-anti-mouse peroxidase conjugated tracer as shown in <b>C</b>. The recombinant and hybridoma-derived antibody showed comparative selectivity- however higher signal intensity. Titration of recombinant and hybridoma derived WO2 against A? 2-42 (<b>D</b>)and A? 3-42(<b>E</b>) indicates that the recombinant antibody has higher activity than the hybridoma-derived counterpart.
<b> Comparison of recombinant WO2 anti-Amyloid beta (24-267) with hybridoma-derived WO2 from Competitor M. </b> Panel <b> A</b> shows a Coomassie Blue-stained SDS-PAGE gel comparing Antibody?s recombinant WO2 with antibody from the corresponding hybridoma under native and reducing (DTT) conditions. Both preparations showed- by this method- comparative purity. Subsequently- the specificity of the antibodies was tested in assays detecting immobilised peptides (see <b>B</b>)- in particular various Amyloid-beta derived peptides probed with WO2 (50 ng/ml) and a goat-anti-mouse peroxidase conjugated tracer as shown in <b>C</b>. The recombinant and hybridoma-derived antibody showed comparative selectivity- however higher signal intensity. Titration of recombinant and hybridoma derived WO2 against A? 2-42 (<b>D</b>)and A? 3-42(<b>E</b>) indicates that the recombinant antibody has higher activity than the hybridoma-derived counterpart.

Documents

Further Information

Additional Names:
Abeta
Buffer:
PBS with 0.02% Proclin 300.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Synthetic peptides, corresponding to amino acids 1-42 of human beta amyloid, conjugated to KLH.
NCBI Gene ID #:
668
NCBI Official Name:
FOXL2
NCBI Official Symbol:
FOXL2
NCBI Organism:
Homo sapiens
Research Area:
Neuroscience
Swissprot #:
P05067
User NOte:
For research use only.