CD3 epsilon Antibody [YTH 12.5], Rabbit IgG

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ProSci
Product Code: PSI-24-204
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-24-204-0.2mg0.2mg£681.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG lambda
Antibody Clonality: Monoclonal
Antibody Clone: YTH 12.5
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
Blue Ice
Storage:
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Images

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<b> Flow-cytometry using anti-CD3 antibody YTH 12.5 (</b> Rhesus monkey lymphocytes were stained with an isotype control (3.0- red) or the rmeric version of YTH 12.5 (24-204- blue) at a concentration of 1 ug/ml for 30 mins at RT. After washing- bound antibody was detected using a AF488 conjugated donkey anti-ribody ( and cells analysed on a FlowJo single-cell flow cytometer.
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<b> Western Blot using anti-CD3E antibody YTH 12.5 (</b> Human spleen sample (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rsion of YTH 12.5 (24-204) at 0.3 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for CD3E is 23.1 kDa. 24-204 successfully detected human CD3E.
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<b> Immunohistochemical staining of human tonsil tissue using anti-CD3E antibody (YTH 12.5 </b> Anti-CD3E staining of paraffin embedded human tissue using the rmeric version of YTH 12.5 (24-204). Antigen retreival was acheived by microwaving in citrate buffer (pH6)- followed by blocking with protein block serum-free buffer (Dako- cat. #X0909). Primary antibody incubation with 24-204 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-r HRP secondary antibody (Dako cat#K4009) for 20 mins followed by Ddiaminobenzidine)- and counter-staining with haemotoxylin. Strong membrane staining of T-cells at the periphery of the germinal centre and between follicles may be observed. Recommended concentration- 2-4 ug/ml.
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<b> Flow-cytometry using anti-CD3 epsilon antibody YTH 12.5 (</b> Human lymphocytes were stained with an isotype control (3.0- panel A) or the rmeric version of YTH 12.5 (24-204- panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing- bound antibody was detected using a AF488 conjugated donkey anti-ribody ( and cells analysed on a FACSCanto flow-cytometer.
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<b> Flow-cytometry using anti-CD3 antibody YTH 12.5 (</b> Rhesus monkey lymphocytes were stained with an isotype control (3.0- red) or the rmeric version of YTH 12.5 (24-204- blue) at a concentration of 1 ug/ml for 30 mins at RT. After washing- bound antibody was detected using a AF488 conjugated donkey anti-ribody ( and cells analysed on a FlowJo single-cell flow cytometer.
<b> Western Blot using anti-CD3E antibody YTH 12.5 (</b> Human spleen sample (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rsion of YTH 12.5 (24-204) at 0.3 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for CD3E is 23.1 kDa. 24-204 successfully detected human CD3E.
<b> Immunohistochemical staining of human tonsil tissue using anti-CD3E antibody (YTH 12.5 </b> Anti-CD3E staining of paraffin embedded human tissue using the rmeric version of YTH 12.5 (24-204). Antigen retreival was acheived by microwaving in citrate buffer (pH6)- followed by blocking with protein block serum-free buffer (Dako- cat. #X0909). Primary antibody incubation with 24-204 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-r HRP secondary antibody (Dako cat#K4009) for 20 mins followed by Ddiaminobenzidine)- and counter-staining with haemotoxylin. Strong membrane staining of T-cells at the periphery of the germinal centre and between follicles may be observed. Recommended concentration- 2-4 ug/ml.
<b> Flow-cytometry using anti-CD3 epsilon antibody YTH 12.5 (</b> Human lymphocytes were stained with an isotype control (3.0- panel A) or the rmeric version of YTH 12.5 (24-204- panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing- bound antibody was detected using a AF488 conjugated donkey anti-ribody ( and cells analysed on a FACSCanto flow-cytometer.

Documents

Further Information

Additional Names:
CD3e, T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, CD3E, T3E, YTH12.5
Buffer:
PBS with 0.02% Proclin 300.
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original rat IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Human CD3.
NCBI Gene ID #:
916
NCBI Official Name:
CD3 epsilon subunit of T-cell receptor complex
NCBI Official Symbol:
CD3E
NCBI Organism:
Homo sapiens
Research Area:
Immunology, Other, Chemokines & Cytokines
Swissprot #:
P07766
User NOte:
For research use only.