TNF (Human) Recombinant Protein
Code | Size | Price |
---|
P7004-100ug | 100 ug | £896.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Human
Regulatory Status: GMP grade
Applications:
- Functional Study
- SDS-PAGE
Storage:
Store at -20°C lyophilized protein is stable for 1 year.
After reconstitution with deionized water store at -20 to -80°C.
Aliquot to avoid repeated freezing and thawing.
After reconstitution with deionized water store at -20 to -80°C.
Aliquot to avoid repeated freezing and thawing.
Further Information
Activity:
The ED50 for this effect is < 0.1 ng/mL, measured by the induction of cytotoxicity in L929 cells in the presence of actinomycin D. The specific activity of recombinant human TNF alpha is approximately > 1 x 10^7 IU/mg, which is calibrated against the human TNF Alpha WHO International Standard (NIBSC code: 12/154).
Endotoxin Level:
< 0.05 EU/ug
Form:
Lyophilized
Gene Alias:
DIF|TNF-alpha|TNFA|TNFSF2
Gene Description:
tumor necrosis factor (TNF superfamily, member 2)
Gene ID:
7124
Gene Name:
TNF
Immunogen Sequence Protein Sequence:
MVRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL
Preparation Method:
Escherichia coli expression system
Product Description:
Human TNF recombinant protein with polyhistidine tag at the C-terminus expressed in Escherichia coli.
Purity:
> 97% by SDS-PAGE
Quality Control Testing:
SDS-PAGE Stained with Coomassie Blue
Reactivity:
Human
Recommend Dilutions:
SDS-PAGE
The optimal working dilution should be determined by the end user.
The optimal working dilution should be determined by the end user.
Storage Buffer:
Lyophilized from a solution containing 1X PBS, pH 8.0.
It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 ug/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.Please use within one month after protein reconstitution.
It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 ug/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.Please use within one month after protein reconstitution.
Tag:
His