anti-CD244 [2B4] (human), mAb (ANC244.8/8F7) (preservative free)

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Ancell
Product Code: ANC-239-820
Product Group: Primary Antibodies
Supplier: Ancell
CodeSizePrice
ANC-239-820-100ug100 ug£243.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Antibody Isotype: Mouse IgG1kappa
Antibody Clonality: Monoclonal
Antibody Clone: ANC244.8/8F7
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Flow Cytometry
Shipping:
BLUE ICE
Storage:
Short term: +4°C. Long term: +4°C

Images

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Binding of anti-CD244 (ANC244.8/8F7) +GAM/FITC to human PBL. Method: Five x 10^5 ficoll prepared human PBMC were washed and incubated 45 minutes on ice with 80ul of anti-CD244 (clone ANC244.8) antibody at 10ug/ml. Cells were washed twice and incubated with 2nd reagent Goat anti-Mouse IgG/FITC (#ANC-232-011), after which they were washed three times, fixed and analyzed by FACS using a lymphocyte gate. A net 43% sub population of cells stained positive with a mean shift of 0.83 log10 fluorescent units when compared to a Mouse IgG1 negative control (#ANC-278-010).
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Binding of anti-CD244 (ANC244.8/8F7) +GAM/FITC to human PBL. Method: Five x 10^5 ficoll prepared human PBMC were washed and incubated 45 minutes on ice with 80ul of anti-CD244 (clone ANC244.8) antibody at 10ug/ml. Cells were washed twice and incubated with 2nd reagent Goat anti-Mouse IgG/FITC (#ANC-232-011), after which they were washed three times, fixed and analyzed by FACS using a lymphocyte gate. A net 43% sub population of cells stained positive with a mean shift of 0.83 log10 fluorescent units when compared to a Mouse IgG1 negative control (#ANC-278-010).

Documents

Further Information

Alternate Names/Synonyms:
Natural Killer Cell Receptor 2B4; NK Cell Activation-inducing Ligand; NAIL; NKR2B4; SLAMF4
Concentration:
1.0mg/ml
EClass:
32160000
Form (Short):
liquid
Formulation:
50mM Sodium Phosphate pH 7.5, 100mM KCl, 150mM NaCl.
Handling Advice:
Avoid freeze/thaw cycles. Keep sterile.
Immunogen:
Recombinant soluble human CD244.
Labels - Conjugates:
Preservative Free
Long Description:
Monoclonal Antibody. Antibody ANC244.8 binds to recombinant CD244-muIg (#ANC-544-020) in EIA, and to cell surface CD244 on human PBL in FACS. ANC244.8 binds to a CD244 epitope that is distinct from the binding sites of clone ANC2B4/3B12 (#ANC-259-020). It blocks binding of clone C1.7. Isotype: Mouse IgG1kappa. Immunogen: Recombinant soluble human CD244. Applications: ELISA, FACS. 50mM Sodium Phosphate pH 7.5, 100mM KCl, 150mM NaCl. CD244 (2B4; SLAMF4) is a 66kDa member of the CD2/SLAM related receptor family (SRR). It is expressed on NK, T cell subsets, monocytes and eosinophils. It is expressed at high levels on leuikemia initiating cells, and plays a role in their proliferative potential. In mice there are two transcription variants, a long form and a short form of CD244 each of which have distinct signaling properties. In humans, only the long form is expressed. Decreased CD244 expression on monocytes of SLE patients correlated (negatively) with autoantibody titer. CD244 mediated function in immune response can vary greatly dependant on the context of its ligation.
NCBI, Uniprot Number:
Q9BZW8
Package Type:
Plastic Vial
Product Description:
CD244 (2B4; SLAMF4) is a 66kDa member of the CD2/SLAM related receptor family (SRR). It is expressed on NK, T cell subsets, monocytes and eosinophils. It is expressed at high levels on leuikemia initiating cells, and plays a role in their proliferative potential. In mice there are two transcription variants, a long form and a short form of CD244 each of which have distinct signaling properties. In humans, only the long form is expressed. Decreased CD244 expression on monocytes of SLE patients correlated (negatively) with autoantibody titer. CD244 mediated function in immune response can vary greatly dependant on the context of its ligation.
Product Line Areas NEW:
Antibody, Cancer, Immunology, Inflammation
Product Type:
Monoclonal Antibody
Purity:
>95% (SDS-PAGE)
Source / Host:
Purified from concentrated hybridoma tissue culture supernatant.
Specificity:
Antibody ANC244.8 binds to recombinant CD244-muIg (#ANC-544-020) in EIA, and to cell surface CD244 on human PBL in FACS. ANC244.8 binds to a CD244 epitope that is distinct from the binding sites of clone ANC2B4/3B12 (#ANC-259-020). It blocks binding of clone C1.7.
Transportation:
Non-hazardous
UNSPSC Category:
Primary Antibodies
UNSPSC Number:
12352203
Use & Stability:
Stable for at least 1 year after receipt when stored at +4A°C.