anti-TIGIT (human), mAb (ANCTG6/10A6) (R-PE)

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Ancell
Product Code: ANC-340-050
Product Group: Primary Antibodies
Supplier: Ancell
CodeSizePrice
ANC-340-050-120tests120 tests£357.00
Quantity:
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Overview

Antibody Isotype: Mouse IgG1kappa
Antibody Clonality: Monoclonal
Antibody Clone: ANCTG6/10A6
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Flow Cytometry
Shipping:
BLUE ICE
Storage:
Short term: +4°C. Long term: +4°C

Images

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Binding of anti-TIGIT/PE to human PBL. Method: Five x 10^5 ficoll prepared human peripheral blood mononuclear cells were washed and pre-incubated 45 minutes on ice with 20?l of 300?g/ml human IgG (to reduce non-specific binding) after which they were incubated with 80?l of anti-TIGIT/PE at a 1:50 (10?g/ml) dilution. Cells were washed three times, fixed and analyzed by FACS using a lymphoid gate. A 36% sub population of the cells stained positive with a mean shift of 0.99 log10 fluorescent units when compared to a Mouse IgG1/R-PE Isotype control (#ANC-278-050). Binding was blocked when cells were pre incubated with 20?l of 0.5 mg/ml anti-TIGIT antibody (#ANC-340-050).
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Binding of anti-TIGIT/PE to human PBL. Method: Five x 10^5 ficoll prepared human peripheral blood mononuclear cells were washed and pre-incubated 45 minutes on ice with 20?l of 300?g/ml human IgG (to reduce non-specific binding) after which they were incubated with 80?l of anti-TIGIT/PE at a 1:50 (10?g/ml) dilution. Cells were washed three times, fixed and analyzed by FACS using a lymphoid gate. A 36% sub population of the cells stained positive with a mean shift of 0.99 log10 fluorescent units when compared to a Mouse IgG1/R-PE Isotype control (#ANC-278-050). Binding was blocked when cells were pre incubated with 20?l of 0.5 mg/ml anti-TIGIT antibody (#ANC-340-050).

Documents

Further Information

Alternate Names/Synonyms:
T Cell Immunoreceptor with Ig and ITIM Domains; V-set and Immunoglobulin Domain-containing Protein 9; V-set and Transmembrane Domain-containing Protein 3; Vstm3; VSIG9; WUCAM
EClass:
32160000
Form (Short):
liquid
Formulation:
50mM Sodium Phosphate pH 7.5, 500mM KCl, 150mM NaCl, 15% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
Handling Advice:
Do not freeze. Protect from light.
Immunogen:
Recombinant human TIGIT.
Labels - Conjugates:
R-PE
Long Description:
Monoclonal Antibody. Binds to receptors on a subset of human peripheral blood lymphoid cells, as well as to recombinant TIGIT in EIA. Isotype: Mouse IgG1kappa. Immunogen: Recombinant human TIGIT. Applications: ELISA, FACS. 50mM Sodium Phosphate pH 7.5, 500mM KCl, 150mM NaCl, 15% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative). TIGIT (T cell immunoreceptor with Ig and ITIM domains) is a novel member of the immunoglobulin super family (IgSF). TIGIT and CD226 bind the same ligands, the two nectin-family members poliovirus receptor (PVR) and poliovirus receptor-related 2 (CD112) and compete for each other. TIGIT inhibits while CD226 activates the signaling through PVR and CD112. TIGIT and CD226 directly interact at the cell surface and this interaction impairs CD226 homodimerization and function. TIGIT is absent on naive T cells, but is expressed on activated Tregs and memory T cells, and on NK cells and NKT cells. TIGIT negatively regulates T cell activation and blockade of TIGIT exacerbates several immune diseases. TIGIT also activates the PVR receptor on dendritic cells (DCs). Addition of TIGIT (human):Fc (human) to DCs triggers phosphorylation of PVR and elicits downstream signaling, which then inhibits T cell responses by producing IL-10. TIGIT also negatively regulates NK cell cytotoxicity and cytokine production. TIGIT is directly regulated by FoxP3 and promotes inducible Treg differentiation.
NCBI, Uniprot Number:
Q495A1
Package Type:
Plastic Vial
Product Description:
TIGIT (T cell immunoreceptor with Ig and ITIM domains) is a novel member of the immunoglobulin super family (IgSF). TIGIT and CD226 bind the same ligands, the two nectin-family members poliovirus receptor (PVR) and poliovirus receptor-related 2 (CD112) and compete for each other. TIGIT inhibits while CD226 activates the signaling through PVR and CD112. TIGIT and CD226 directly interact at the cell surface and this interaction impairs CD226 homodimerization and function. TIGIT is absent on naive T cells, but is expressed on activated Tregs and memory T cells, and on NK cells and NKT cells. TIGIT negatively regulates T cell activation and blockade of TIGIT exacerbates several immune diseases. TIGIT also activates the PVR receptor on dendritic cells (DCs). Addition of TIGIT (human):Fc (human) to DCs triggers phosphorylation of PVR and elicits downstream signaling, which then inhibits T cell responses by producing IL-10. TIGIT also negatively regulates NK cell cytotoxicity and cytokine production. TIGIT is directly regulated by FoxP3 and promotes inducible Treg differentiation.
Product Line Areas NEW:
Antibody, Cancer, Immunology, Immuno-Oncology
Product Type:
Monoclonal Antibody
Purity:
Protein A purified.
Source / Host:
Purified from concentrated hybridoma tissue culture supernatant.
Specificity:
Binds to receptors on a subset of human peripheral blood lymphoid cells, as well as to recombinant TIGIT in EIA.
Transportation:
Non-hazardous
UNSPSC Category:
Primary Antibody Conjugates
UNSPSC Number:
12352203
Use & Stability:
Stable for at least 1 year after receipt when stored at +4A°C.