anti-CD8-alpha (mouse), mAb (53-6) (preservative free)

Contact us
Ancell
Product Code: ANC-260-820
Product Group: Primary Antibodies
Supplier: Ancell
CodeSizePrice
ANC-260-820-100ug100 ug£208.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Antibody Isotype: Rat IgG2akappa
Antibody Clonality: Monoclonal
Antibody Clone: 53-6
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Flow Cytometry
Shipping:
BLUE ICE
Storage:
Short term: +4°C. Long term: +4°C

Images

1 / 1
Binding of anti-mCD8alpha (53-6.72) +GAR/FITC to balb-c splenocytes. Method: Tested for binding to ACK lysed murine splenocytes in FACS. Five x 10^5 splenocytes per tube were washed and pre-incubated with 20ul of 300ug/ml murine IgG (to reduce non-specific binding) after which they were incubated 45 minutes on ice with 80ul of anti-CD8 antibody diluted to 10ug/ml. Cells were washed twice and incubated with 2nd reagent Goat anti-Rat IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. An 8.8 % sub population of the cells stained positive with a mean shift of 1.6 log10 fluorescent units when compared to background.
❮❯

Binding of anti-mCD8alpha (53-6.72) +GAR/FITC to balb-c splenocytes. Method: Tested for binding to ACK lysed murine splenocytes in FACS. Five x 10^5 splenocytes per tube were washed and pre-incubated with 20ul of 300ug/ml murine IgG (to reduce non-specific binding) after which they were incubated 45 minutes on ice with 80ul of anti-CD8 antibody diluted to 10ug/ml. Cells were washed twice and incubated with 2nd reagent Goat anti-Rat IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. An 8.8 % sub population of the cells stained positive with a mean shift of 1.6 log10 fluorescent units when compared to background.

Documents

Further Information

Alternate Names/Synonyms:
T-cell Surface Glycoprotein CD8 alpha Chain
Concentration:
1mg/ml
EClass:
32160000
Form (Short):
liquid
Formulation:
50 mM Sodium Phosphate pH 7.5, 100mM KCl, 150mM NaCl.
Handling Advice:
Avoid freeze/thaw cycles.
Immunogen:
Murine thymocytes/splenocytes.
Labels - Conjugates:
Preservative Free
Long Description:
Monoclonal Antibody. Isotype: Rat IgG2akappa. Immunogen: Murine thymocytes/splenocytes. Applications: ELISA, FACS. 50 mM Sodium Phosphate pH 7.5, 100mM KCl, 150mM NaCl. CD8 identifies cytotoxic/suppressor T cells that interact with MHC Class I bearing targets. CD8 is thought to play a role in the process of T cell mediated killing. CD8-alpha chains binds to Class I MHC molecules alpha-3 domains. Defects in CD8A are a cause of familial CD8 deficiency. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
NCBI, Uniprot Number:
P01731
Package Type:
Plastic Vial
Product Description:
CD8 identifies cytotoxic/suppressor T cells that interact with MHC Class I bearing targets. CD8 is thought to play a role in the process of T cell mediated killing. CD8-alpha chains binds to Class I MHC molecules alpha-3 domains. Defects in CD8A are a cause of familial CD8 deficiency. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
Product Line Areas NEW:
Antibody, Immunology, Inflammation
Product Type:
Monoclonal Antibody
Purity:
>95% (SDS-PAGE)
Source / Host:
Purified from concentrated hybridoma tissue culture supernatant.
Transportation:
Non-hazardous
UNSPSC Category:
Primary Antibodies
UNSPSC Number:
12352203
Use & Stability:
Stable for at least 1 year after receipt when stored at +4A°C.