Boc-Val-Pro-Arg-AMC . HCl
| Code | Size | Price |
|---|
| AG-CP3-0038-M005 | 5 mg | £80.00 |
Quantity:
| AG-CP3-0038-M025 | 25 mg | £290.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Shipping:
AMBIENT
Storage:
Short term: +4°C. Long term: -20°C
Documents
Further Information
Alternate Names/Synonyms:
Boc-VPR-AMC . HCl
Appearance:
Solid lyophilized powder.
CAS:
70375-24-5 | 65147-04-8 (free base)
EClass:
32160000
Form (Short):
solid
Handling Advice:
Avoid freeze/thaw cycles. Protect from light.
InChi:
InChI=1S/C31H45N7O7.ClH/c1-17(2)25(37-30(43)45-31(4,5)6)28(42)38-14-8-10-22(38)27(41)36-21(9-7-13-34-29(32)33)26(40)35-19-11-12-20-18(3)15-24(39)44-23(20)16-19;/h11-12,15-17,21-22,25H,7-10,13-14H2,1-6H3,(H,35,40)(H,36,41)(H,37,43)(H4,32,33,34);1H
InChiKey:
ROQGJTZGLVLVIN-UHFFFAOYSA-N
Long Description:
Chemical. CAS: 70375-24-5 | 65147-04-8 (free base). Formula: C31H45N7O7 . HCl. MW: 627.7 . 36.5. Boc-Val-Pro-Arg-AMC is a sensitive fluorogenic peptide substrate for α-thrombin (Km = 21 uM, kcat = 109 s-1) and the α-thrombin-staphylocoagulase complex (Km = 25 uM, kcat = 89 s-1). It also serves as a substrate vor Kallikrein 5 and 8. Activity is quantified by the release of free fluorescent 7-amino-4-methylcoumarin (AMC) which excites at 360-380nm and emits at 440-460nm when interacting with a target enzyme. This substrate has also been used for the rapid detection of MRSA (methicillin-resistant Staphylococcus aureus).
MDL:
MFCD00058181
Molecular Formula:
C31H45N7O7 . HCl
Molecular Weight:
627.7 . 36.5
Package Type:
Vial
Product Description:
Boc-Val-Pro-Arg-AMC is a sensitive fluorogenic peptide substrate for α-thrombin (Km = 21 µM, kcat = 109 s-1) and the α-thrombin-staphylocoagulase complex (Km = 25 µM, kcat = 89 s-1). It also serves as a substrate vor Kallikrein 5 and 8. Activity is quantified by the release of free fluorescent 7-amino-4-methylcoumarin (AMC) which excites at 360-380nm and emits at 440-460nm when interacting with a target enzyme. This substrate has also been used for the rapid detection of MRSA (methicillin-resistant Staphylococcus aureus).
Product Line Areas NEW:
Biochemicals, Cancer, Immunology
Product Type:
Chemical
Purity:
>98%
Sequence:
Boc-Val-Pro-Arg-7-amido-4-methylcoumarin
SMILES:
O=C1C=C(C)C2=CC=C(NC(C(CCCNC(N)=N)NC(C3N(C(C(NC(OC(C)(C)C)=O)C(C)C)=O)CCC3)=O)=O)C=C2O1.Cl
Solubility Chemicals:
Soluble in water (10mg/ml). Soluble in 5% acetic acid.
Transportation:
Non-hazardous
UNSPSC Category:
Biochemical Reagents
UNSPSC Number:
12352200
Use & Stability:
Stable for at least 1 year after receipt when stored at -20°C.
References
New fluorogenic substrates for alpha-thrombin, factor Xa, kallikreins, and urokinase: T. Morita, et al.; J. Biochem. 82, 1495 (1977) | Difference in enzymatic properties between alpha-thrombin-staphylocoagulase complex and free alpha-thrombin: S. Kawabata, et al.; J. Biochem. 97, 1073 (1985) | Evaluation of tube coagulase and a fluorogenic substrate for rapid detection of methicillin-resistant Staphylococcus aureus from selective enrichment broth in an outbreak of EMRSA 15: M. Ford, et al.; J. Hosp. Infect. 41, 133 (1999) | Biochemical and Enzymatic Characterization of Human Kallikrein 5 (hK5), a Novel Serine Protease Potentially Involved in Cancer Progression: I.P. Michael, et al.; J. Biol. Chem. 280, 14628 (2005) | Activation and enzymatic characterization of recombinant human kallikrein 8: T. Kishi, et al.; Biol. Chem. 387, 723 (2006)