Human ALDH9A1(Aldehyde Dehydrogenase 9 Family, Member A1) ELISA Kit

ELK Biotechnology
Product Code: ELK9232
Product Group: ELISA Kits
CodeSizePrice
ELK9232-48T48T£298.00
Quantity:
ELK9232-96T96T£390.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Regulatory Status: RUO
Target Species: Human
Application: Enzyme-Linked Immunosorbent Assay (ELISA)

Video

Further Information

Alternative Names:
ALDH4; ALDH7; ALDH9; E3; TMABADH; 4-Trimethylaminobutyraldehyde Dehydrogenase; Aldehyde dehydrogenase E3 isozyme; Gamma-aminobutyraldehyde dehydrogenase
Assay length:
3.5h
Assay Type:
Sandwich
Detection range:
0.16-10 ng/mL
Research Area:
Metabolic pathway;
Sample type:
Serum, plasma, tissue homogenates and other biological fluids.
Sensitivity:
0.066 ng/mL
Standard:
10 ng/mL
Test principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aldehyde Dehydrogenase 9 Family, Member A1(ALDH9A1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Aldehyde Dehydrogenase 9 Family, Member A1(ALDH9A1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aldehyde Dehydrogenase 9 Family, Member A1(ALDH9A1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Aldehyde Dehydrogenase 9 Family, Member A1(ALDH9A1) in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID:
P49189