Human MTOR(Serine/threonine-protein kinase mTOR) ELISA Kit
| Code | Size | Price |
|---|
| ELK9237-48T | 48T | £298.00 |
Quantity:
| ELK9237-96T | 96T | £390.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
FRAP; FRAP1; FRAP2; MTOR; RAFT1; RAPT1; FK506 Binding Protein 12 Rapamycin Associated Protein; Mammalian Target Of Rapamycin; FKBP12-Rapamycin Complex-Associated Protein 1; Rapamycin and FKBP12 target
Assay length:
3.5h
Assay Type:
Sandwich
Detection range:
0.32-20 ng/mL
Research Area:
Cell proliferation
Sample type:
serum, plasma, tissue homogenates and other biological fluids
Sensitivity:
0.125 ng/mL
Standard:
20 ng/mL
Test principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serine/threonine-protein kinase mTOR(MTOR). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Serine/threonine-protein kinase mTOR(MTOR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Serine/threonine-protein kinase mTOR(MTOR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Serine/threonine-protein kinase mTOR(MTOR) in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID:
P42345