Cattle TRH(Thyrotropin Releasing Hormone) ELISA Kit
| Code | Size | Price |
|---|
| ELK9239-48T | 48T | £298.00 |
Quantity:
| ELK9239-96T | 96T | £390.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Bovine (Cattle)
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
TRF; Thyrotropin-Releasing Factor; Thyroliberin; Protirelin; Pro-tirelin; Prothyroliberin; TSH-releasing factor
Assay length:
2h
Assay Type:
Competitive Inhibition
Detection range:
7.82-500 pg/mL
Research Area:
Endocrinology;Neuro science;Hepatology;Hormone metabolism;
Sample type:
serum, plasma and other biological fluids
Sensitivity:
2.63 pg/mL
Standard:
500 pg/mL
Test principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Thyrotropin Releasing Hormone(Cattle TRH) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Thyrotropin Releasing Hormone(Cattle TRH). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thyrotropin Releasing Hormone(Cattle TRH) in the samples is then determined by comparing the OD of the samples to the standard curve.