Human ACA-IgM(Anti-Cardiolipin Antibody IgM) ELISA Kit
| Code | Size | Price |
|---|
| ELK9254-48T | 48T | £298.00 |
Quantity:
| ELK9254-96T | 96T | £390.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
ACA-IgM; Anti-Cardiolipin Antibody IgM
Assay length:
3.5h
Assay Type:
Sandwich
Detection range:
0.31-20 ng/mL
Research Area:
Cardiovascular biology
Sample type:
serum, plasma and other biological fluids
Sensitivity:
0.082 ng/mL
Standard:
20 ng/mL
Test principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Anti-Cardiolipin Antibody IgM(ACA-IgM). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Anti-Cardiolipin Antibody IgM(ACA-IgM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Anti-Cardiolipin Antibody IgM(ACA-IgM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-Cardiolipin Antibody IgM(ACA-IgM) in the samples is then determined by comparing the OD of the samples to the standard curve.