Chicken 8-iso-PGF2α(8-isoprostane) ELISA Kit
| Code | Size | Price |
|---|
| ELK9298-48T | 48T | £298.00 |
Quantity:
| ELK9298-96T | 96T | £390.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Chicken (Gallus)
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
8-isoprostane; 8-iso-PGF2α
Assay length:
2h
Assay Type:
Competitive Inhibition
Detection range:
15.625-1000 pg/mL
Research Area:
Endocrinology;Pulmonology;Hepatology;Hormone metabolism;
Sample type:
serum, plasma and other biological fluids
Sensitivity:
9.375 pg/mL
Standard:
1000 pg/mL
Test principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 8-isoprostane(8-iso-PGF2α) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 8-isoprostane(8-iso-PGF2α). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 8-isoprostane(8-iso-PGF2α) in the samples is then determined by comparing the OD of the samples to the standard curve.