Chicken IDO(Indoleamine-2,3-Dioxygenase) ELISA Kit
| Code | Size | Price |
|---|
| ELK9299-48T | 48T | £298.00 |
Quantity:
| ELK9299-96T | 96T | £390.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Chicken (Gallus)
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
CD107B; INDO; Indoleamine-Pyrrole 2,3 Dioxygenase
Assay length:
3.5h
Assay Type:
Sandwich
Detection range:
0.16-10 ng/mL
Research Area:
Enzyme & Kinase;Infection immunity;
Sample type:
serum, plasma, tissue homogenates and other biological fluids
Sensitivity:
0.057 ng/mL
Standard:
10 ng/mL
Test principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Indoleamine-2,3-Dioxygenase(IDO). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Indoleamine-2,3-Dioxygenase(IDO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Indoleamine-2,3-Dioxygenase(IDO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Indoleamine-2,3-Dioxygenase(IDO) in the samples is then determined by comparing the OD of the samples to the standard curve.