Human pNF-H(Phosphorylated Neurofilament H) ELISA Kit
| Code | Size | Price |
|---|
| ELK9302-48T | 48T | £298.00 |
Quantity:
| ELK9302-96T | 96T | £390.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
TC2; Transcobalamin II
Assay length:
3.5h
Assay Type:
Sandwich
Detection range:
78.13-5000 pg/mL
Research Area:
Neuro science
Sample type:
Serum, plasma, tissue homogenates and other biological fluids.
Sensitivity:
29 pg/mL
Standard:
5000 pg/mL
Test principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphorylated Neurofilament H(pNF-H). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Phosphorylated Neurofilament H(pNF-H). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phosphorylated Neurofilament H(pNF-H), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phosphorylated Neurofilament H(pNF-H) in the samples is then determined by comparing the OD of the samples to the standard curve.