Mouse COX1(Cytochrome C Oxidase Subunit I) ELISA Kit
| Code | Size | Price |
|---|
| ELK9311-48T | 48T | £298.00 |
Quantity:
| ELK9311-96T | 96T | £390.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Mouse
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
MT-CO1; MTCO1; COI; COXI; Mitochondrially Encoded Cytochrome C Oxidase I; Cytochrome c oxidase polypeptide I
Assay length:
3.5h
Assay Type:
Sandwich
Detection range:
0.32-20 ng/mL
Research Area:
Enzyme & Kinase;
Sample type:
Tissue homogenates, cell lysates and other biological fluids.
Sensitivity:
0.122 ng/mL
Standard:
20 ng/mL
Test principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome C Oxidase Subunit I(COX1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cytochrome C Oxidase Subunit I(COX1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome C Oxidase Subunit I(COX1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytochrome C Oxidase Subunit I(COX1) in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID:
P00397