Human PGF2a(Prostaglandin F2 Alpha) ELISA Kit
| Code | Size | Price |
|---|
| ELK9314-48T | 48T | £261.00 |
Quantity:
| ELK9314-96T | 96T | £335.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
PGF2A; PGF2-A; PG-F2a
Assay length:
2h
Assay Type:
Competitive Inhibition
Detection range:
12.5-800 pg/mL
Research Area:
Metabolic pathway;Cardiovascular biology;Hormone metabolism;
Sample type:
serum, plasma and other biological fluids
Sensitivity:
3.88 pg/mL
Standard:
800 pg/mL
Test principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Prostaglandin F2 Alpha(PGF2a) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Prostaglandin F2 Alpha(PGF2a). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Prostaglandin F2 Alpha(PGF2a) in the samples is then determined by comparing the OD of the samples to the standard curve.