Cat SDMA(Symmetric dimethylarginine) ELISA Kit

ELK Biotechnology
Product Code: ELK9318
Product Group: ELISA Kits
CodeSizePrice
ELK9318-48T48T£298.00
Quantity:
ELK9318-96T96T£390.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Regulatory Status: RUO
Target Species: Feline (cat)
Application: Enzyme-Linked Immunosorbent Assay (ELISA)

Video

Further Information

Alternative Names:
Symmetric dimethylarginine
Assay length:
2h
Assay Type:
Competitive Inhibition
Detection range:
0.16-10 ng/mL
Research Area:
Signal transduction;Developmental science;Bone metabolism;
Sample type:
tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity:
0.051 ng/mL
Standard:
10 ng/mL
Test principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Symmetric dimethylarginine(SDMA) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Symmetric dimethylarginine(SDMA). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Symmetric dimethylarginine(SDMA) in the samples is then determined by comparing the OD of the samples to the standard curve.