Sheep vWF(Von Willebrand Factor) ELISA Kit
| Code | Size | Price |
|---|
| ELK9355-48T | 48T | £359.00 |
Quantity:
| ELK9355-96T | 96T | £477.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Sheep
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
F8VWF; VWD; von Willebrand antigen 2
Assay length:
2h
Assay Type:
Competitive Inhibition
Detection range:
12.5-800 ng/mL
Research Area:
Signal transduction;Hematology;
Sample type:
Plasma.
Sensitivity:
4.11 ng/mL
Standard:
800 ng/mL
Test principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Von Willebrand Factor(vWF) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Von Willebrand Factor(vWF). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Von Willebrand Factor(vWF) in the samples is then determined by comparing the OD of the samples to the standard curve.