Pig BMG/b2-MG(Beta-2-Microglobulin) ELISA Kit
| Code | Size | Price |
|---|
| ELK9425-48T | 48T | £359.00 |
Quantity:
| ELK9425-96T | 96T | £477.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Porcine (pig)
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
Beta 2M; BMG; B2MG; b2M; Beta-2-Microglobulin
Assay length:
3.5h
Assay Type:
Sandwich
Detection range:
3.13-200 ng/mL
Research Area:
Infection immunity;Kidney biomarker;
Sample type:
Serum, plasma, urine and other biological fluids
Sensitivity:
1.19 ng/mL
Standard:
200 ng/mL
Test principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Beta-2-Microglobulin(BMG/b2-MG). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Beta-2-Microglobulin(BMG/b2-MG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Beta-2-Microglobulin(BMG/b2-MG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Beta-2-Microglobulin(BMG/b2-MG) in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID:
Q07717