Horse cTnT/TNNT2(Troponin T Type 2, Cardiac) ELISA Kit
| Code | Size | Price |
|---|
| ELK9455-48T | 48T | £359.00 |
Quantity:
| ELK9455-96T | 96T | £477.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Equine (Horse)
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
CMH2; cTnT; TnTc; Cardiomyopathy,Hypertrophic 2; Cardiac muscle troponin T; TNNT2; Troponin T Type 2, Cardiac
Assay length:
3.5h
Assay Type:
Sandwich
Detection range:
15.63-1000 pg/mL
Research Area:
Cardiovascular biology;
Sample type:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity:
6 pg/mL
Standard:
1000 pg/mL
Test principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Troponin T Type 2, Cardiac(cTnT/TNNT2). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Troponin T Type 2, Cardiac(cTnT/TNNT2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Troponin T Type 2, Cardiac(cTnT/TNNT2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Troponin T Type 2, Cardiac(cTnT/TNNT2) in the samples is then determined by comparing the OD of the samples to the standard curve.