Human LNGFR(Low Affinity Nerve Growth Factor Receptor) ELISA Kit
| Code | Size | Price |
|---|
| ELK9458-48T | 48T | £298.00 |
Quantity:
| ELK9458-96T | 96T | £390.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
CD271; NGFR; TNFRSF16; P75(NTR); Tumor necrosis factor receptor superfamily member 16; P75 Neurotrophin Receptor; Gp80-LNGFR; Low affinity neurotrophin receptor p75NTR
Assay length:
3.5h
Assay Type:
Sandwich
Detection range:
0.16-10 ng/mL
Research Area:
Signal transduction;Infection immunity;Neuro science;
Sample type:
Serum, plasma, tissue homogenates and other biological fluids
Sensitivity:
0.058 ng/mL
Standard:
10 ng/mL
Test principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Low Affinity Nerve Growth Factor Receptor(LNGFR). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Low Affinity Nerve Growth Factor Receptor(LNGFR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Low Affinity Nerve Growth Factor Receptor(LNGFR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Low Affinity Nerve Growth Factor Receptor(LNGFR) in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID:
P08138